Institut für Biologie, Martin-Luther-Universität Halle-Wittenberg, Hoher Weg 4, D-06099 Halle/Saale, Germany.
Mol Ecol Resour. 2008 Sep;8(5):1034-6. doi: 10.1111/j.1755-0998.2008.02146.x. Epub 2008 Jun 28.
We present a set of 18 microsatellite DNA markers that can be run in two multiplex polymerase chain reactions as standard tool for assessing molecular ecological problems in honeybees (Apis mellifera). In addition to a set of six unlinked loci testing for classical population genetic parameters, we present three sets of four tightly linked loci, each located on three different chromosomes. These linked markers are useful for determining the number of colonies in a population as well as the parentage of drones and workers. Moreover, the tool kit can test for various modes of natural selection in honeybee populations.
我们提出了一套 18 个微卫星 DNA 标记物，这些标记物可以在两个多重聚合酶链反应中运行，作为评估蜜蜂（Apis mellifera）分子生态学问题的标准工具。除了一组用于测试经典群体遗传参数的六个不连锁的基因座外，我们还提出了三套四个紧密连锁的基因座，每个基因座位于三个不同的染色体上。这些连锁标记物可用于确定种群中的殖民地数量以及雄蜂和工蜂的亲缘关系。此外，这个工具包还可以测试蜜蜂种群中各种自然选择模式。
