开发并鉴定了九对用于墨西哥蟾蜍（Spea multiplicata）的多态微卫星标记，这些标记在平原蟾蜍（S. bombifrons）中也可进行交叉扩增。

Development and characterization of nine polymorphic microsatellite markers for Mexican spadefoot toads (Spea multiplicata) with cross-amplification in Plains spadefoot toads (S. bombifrons).

机构信息

Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA, Southwest Fisheries Science Center, 110 Shaffer Road, Santa Cruz, CA 95060, USA, Department of Biology, University of North Dakota, Grand Forks, ND 58202, USA.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1386-9. doi: 10.1111/j.1755-0998.2008.02291.x.

DOI:10.1111/j.1755-0998.2008.02291.x

PMID:21586053

Abstract

We developed nine polymorphic microsatellite markers for the Mexican spadefoot toad, Spea multiplicata. Allele numbers range from five to 12, with observed heterozygosities from 0.48 to 0.87. Because two loci are in linkage disequilibrium, these nine loci provide eight independent markers. Three loci exhibit departure from Hardy-Weinberg equilibrium, possibly resulting from null alleles or population admixture. These markers will be useful for assessing population structure and relatedness in S. multiplicata. Based on our success at cross-amplification in the Plains spadefoot toad (Spea bombifrons), these loci also may be useful in this species with additional optimization.

摘要

我们为墨西哥蟾蜍 Spea multiplicata 开发了九个多态性微卫星标记。等位基因数从 5 到 12 不等，观察到的杂合度从 0.48 到 0.87。由于两个位点处于连锁不平衡状态，因此这九个位点提供了八个独立的标记。三个位点偏离哈迪-温伯格平衡，可能是由于无效等位基因或种群混合造成的。这些标记将有助于评估 S. multiplicata 的种群结构和相关性。基于我们在平原蟾蜍（Spea bombifrons）中的交叉扩增成功，这些位点在经过额外优化后也可能对该物种有用。