Yatkin Emrah, Daglioglu Suzan
Department of Histology and Embryology, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey.
J Vet Sci. 2011 Jun;12(2):121-6. doi: 10.4142/jvs.2011.12.2.121.
This study was designed to investigate the potential estrogenic effects of perinatal dietary phytoestrogens on the rat uterus. Pregnant rats were divided to three groups provided the following diets: (1) rat chow, (2) rat chow with 7.5% Trifolium (T.) pratense, or (3) rat chow supplemented with 17β-estradiol (0.5 mg/kg). The dams in each group were kept on the same diet during pregnancy and lactation. Female offspring were euthanized on day 21 at which time body and organ weights were recorded and tissue samples were taken for histology. Immunohistochemistry was performed to detect estrogen receptor alpha (ERα) and progesterone receptor (PR) levels. Our results revealed estrogen-like biological effects of perinatal T. pratense exposure. Relative uterus and ovary weights in the experimental groups were increased compared to control. The number of uterine glands and luminal epithelium heights were also increased. However, there were no statistically significant changes detected in the immunostaining intensity of ERα and PR between the groups.
本研究旨在调查围产期膳食植物雌激素对大鼠子宫的潜在雌激素样作用。将怀孕大鼠分为三组，分别给予以下饮食：(1) 大鼠饲料，(2) 添加7.5% 红车轴草的大鼠饲料，或(3) 添加17β-雌二醇（0.5 mg/kg）的大鼠饲料。每组母鼠在怀孕和哺乳期均给予相同的饮食。雌性后代在第21天安乐死，此时记录体重和器官重量，并采集组织样本进行组织学检查。采用免疫组织化学法检测雌激素受体α（ERα）和孕激素受体（PR）水平。我们的结果显示围产期暴露于红车轴草具有雌激素样生物学效应。与对照组相比，实验组的相对子宫和卵巢重量增加。子宫腺数量和管腔上皮高度也增加。然而，各组之间ERα和PR的免疫染色强度未检测到统计学上的显著变化。
