Robbins S G, Hamel C P, Detrick B, Hooks J J
Immunology and Virology Section, National Eye Institute, National Institutes of Health.
Lab Invest. 1990 Apr;62(4):417-26.
The ability of the coronavirus mouse hepatitis virus, strain JHM, to grow in the retinas of BALB/c mice was examined. Inoculation into the vitreous chamber produced significant changes. Immunoperoxidase staining of frozen sections with either monoclonal or polyclonal antiserum revealed coronaviral antigens in the iris, ciliary body, and a few ganglion cells on day 1. The retinal pigment epithelial cells began expressing viral antigen on day 2 and large amounts of antigen were present in these cells on day 3. Viral antigens were detected in all layers of the neural retina by day 6 and were absent after day 7. Infectious virus was recovered from retinas harvested at 5 days. The drop in viral antigen expression was correlated with an elevation in virus-specific antibody; the latter began to rise on day 5 and plateaued after day 8. In hematoxylin and eosin- or periodic acid -Schiff-stained sections of virus-inoculated left eyes, but not mock-inoculated right eyes, lesions spanning all layers of the neural retina were detected by day 3. Subsequently abnormalities in retinal pigment epithelial cells appeared, sometimes around the entire circumference of the retina. Significant retinal abnormalities, notably photoreceptor degeneration, persisted through 6 weeks. These results demonstrate that coronaviruses can cause acute infection of the posterior pole of the eye, resulting in only a mild inflammatory response and long-lasting disease. This murine disease may be considered a model for degenerative diseases of the pigment epithelium and photoreceptors in man.
研究了冠状病毒小鼠肝炎病毒JHM株在BALB/c小鼠视网膜中的生长能力。向玻璃体腔接种病毒产生了显著变化。用单克隆或多克隆抗血清对冰冻切片进行免疫过氧化物酶染色，结果显示在第1天，虹膜、睫状体和少数神经节细胞中存在冠状病毒抗原。视网膜色素上皮细胞在第2天开始表达病毒抗原，第3天这些细胞中存在大量抗原。到第6天，在神经视网膜的所有层中都检测到了病毒抗原，第7天后则未检测到。在第5天收获的视网膜中回收了感染性病毒。病毒抗原表达的下降与病毒特异性抗体的升高相关；后者在第5天开始上升，第8天后达到稳定水平。在苏木精-伊红或过碘酸-希夫染色的接种病毒的左眼切片中，但未接种病毒的右眼切片中未检测到，在第3天检测到跨越神经视网膜所有层的病变。随后，视网膜色素上皮细胞出现异常，有时围绕视网膜的整个圆周。明显的视网膜异常，尤其是光感受器变性，持续了6周。这些结果表明，冠状病毒可引起眼后极的急性感染，仅导致轻度炎症反应和持久的疾病。这种小鼠疾病可被视为人类色素上皮和光感受器退行性疾病的模型。
