Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, Patricia A. Sullivan Science Building, PO Box 26170, Greensboro, NC, 27402, USA.
Phytochem Anal. 2013 Nov-Dec;24(6):654-60. doi: 10.1002/pca.2449. Epub 2013 Jun 18.
Grapefruit juice can increase or decrease the systemic exposure of myriad oral medications, leading to untoward effects or reduced efficacy. Furanocoumarins in grapefruit juice have been established as inhibitors of cytochrome P450 3A (CYP3A)-mediated metabolism and P-glycoprotein (P-gp)-mediated efflux, while flavonoids have been implicated as inhibitors of organic anion transporting polypeptide (OATP)-mediated absorptive uptake in the intestine. The potential for drug interactions with a food product necessitates an understanding of the expected concentrations of a suite of structurally diverse and potentially bioactive compounds.
Develop methods for the rapid quantitation of two furanocoumarins (bergamottin and 6',7'-dihydroxybergamottin) and four flavonoids (naringin, naringenin, narirutin and hesperidin) in five grapefruit juice products using ultra-performance liquid chromatography (UPLC).
Grapefruit juice products were extracted with ethyl acetate; the concentrated extract was analysed by UPLC using acetonitrile:water gradients and a C18 -column. Analytes were detected using a photodiode array detector, set at 250 nm (furanocoumarins) and 310 nm (flavonoids). Intraday and interday precision and accuracy and limits of detection and quantitation were determined.
Rapid (< 5.0 min) UPLC methods were developed to measure the aforementioned furanocoumarins and flavonoids. R(2) values for the calibration curves of all analytes were >0.999. Considerable between-juice variation in the concentrations of these compounds was observed, and the quantities measured were in agreement with the concentrations published in HPLC studies.
These analytical methods provide an expedient means to quantitate key furanocoumarins and flavonoids in grapefruit juice and other foods used in dietary substance-drug interaction studies.
葡萄柚汁可增加或减少无数口服药物的全身暴露，导致不良反应或疗效降低。葡萄柚汁中的呋喃香豆素已被确定为细胞色素 P450 3A（CYP3A）介导的代谢和 P-糖蛋白（P-gp）介导的外排的抑制剂，而类黄酮则被认为是肠内有机阴离子转运多肽（OATP）介导的吸收摄取的抑制剂。由于一种食物产品可能会与药物发生相互作用，因此需要了解一套结构多样且具有潜在生物活性的化合物的预期浓度。
开发使用超高效液相色谱法（UPLC）快速定量五种葡萄柚汁产品中的两种呋喃香豆素（佛手柑素和 6'，7'-二羟基佛手柑素）和四种类黄酮（柚皮苷、柚皮苷、橙皮苷和橙皮苷）的方法。
用乙酸乙酯提取葡萄柚汁产品；浓缩提取物通过 UPLC 进行分析，采用乙腈：水梯度和 C18 柱。使用光电二极管阵列检测器在 250nm（呋喃香豆素）和 310nm（类黄酮）处检测分析物。测定日内和日间精密度和准确度以及检测限和定量限。
开发了快速（<5.0min）UPLC 方法来测量上述呋喃香豆素和类黄酮。所有分析物的校准曲线的 R（2）值均>0.999。这些化合物在不同果汁之间的浓度存在很大差异，并且测量的数量与 HPLC 研究中公布的浓度一致。
这些分析方法为定量葡萄柚汁和其他用于膳食物质-药物相互作用研究的食品中的关键呋喃香豆素和类黄酮提供了一种快捷的方法。
