Gupta V, Rajaraman S, Eberle R
Division of Medical Oncology, Pittsburgh Cancer Institute, PA.
Carcinogenesis. 1990 May;11(5):713-22. doi: 10.1093/carcin/11.5.713.
This report provides evidence for spontaneous transfer of human tumor DNA in vivo to mouse cells by a human small cell lung cancer implanted in nude mice. The transformation of the mouse cells was characterized by the presence of neurosecretory granules, which are hallmarks of small cell lung cancer. The carcinogenicity of the human xenografted tumor DNA was confirmed by transfecting NIH3T3 cells in vitro, suggesting that the human tumor DNA may have been the cause of the transformation of the mouse cells in vivo. The spontaneous induction of malignancy in mouse cells in vivo by human tumor DNA may be a factor of potential importance in tumor cell heterogeneity and propagation of the malignant state for some tumors. The observations support the thesis that some human cancer cells can transform normal cells to become malignant in vivo.
本报告提供了证据，证明植入裸鼠体内的人小细胞肺癌可在体内将人肿瘤DNA自发转移至小鼠细胞。小鼠细胞的转化特征为存在神经分泌颗粒，这是小细胞肺癌的标志。通过体外转染NIH3T3细胞证实了人异种移植肿瘤DNA的致癌性，这表明人肿瘤DNA可能是体内小鼠细胞转化的原因。人肿瘤DNA在体内自发诱导小鼠细胞发生恶性转化，可能是某些肿瘤中肿瘤细胞异质性和恶性状态传播的一个潜在重要因素。这些观察结果支持了这样一种观点，即某些人类癌细胞可在体内将正常细胞转化为恶性细胞。
