Szalay S, Leodolter S, Lubec G
Onkologie. 1978 Oct;1(5):194-6. doi: 10.1159/000213950.
We estimated collagenolytic activity of 10 invasive carcinomas of the cervix by means of a biological assay. Collagenolysis was found in 7 specimens. In order to detect the origin of the collagenolytic enzyme additional inhibition studies with ethylendiaminetetraacetate and normal human serum were performed. Normal human serum inhibited the enzymatic process by a mean percentage of 14.9%, EDTA up to 9.1%. D-penicillamine and 1.10-o-phenanthroline which inhibit collagenase specifically stopped the enzymatic activity of the tumor completely. According to our results it can be suggested that the collagenolytic activity of carcinoma of the cervix uteri is not derived from serum or from polymorphonuclear cells but is created by the tumor itself.
我们通过生物测定法估算了10例宫颈浸润癌的胶原溶解活性。在7个标本中发现了胶原溶解现象。为了检测胶原溶解酶的来源，我们用乙二胺四乙酸和正常人血清进行了额外的抑制研究。正常人血清平均抑制酶促过程的比例为14.9%，乙二胺四乙酸高达9.1%。特异性抑制胶原酶的D-青霉胺和1,10-邻菲啰啉完全阻断了肿瘤的酶活性。根据我们的结果，可以认为子宫颈癌的胶原溶解活性并非源自血清或多形核细胞，而是由肿瘤自身产生的。
