Lubec G, Möschl P, Keiler A, Szalay S
Pathol Res Pract. 1980;167(2-4):287-91. doi: 10.1016/S0344-0338(80)80058-6.
Six mongrel dogs were presensitized by full thickness skin grafts followed by orthotopic lung homotransplantation. Tissue explants of the hyperacutely rejected lung grafts were placed on the surface of a gel of native collagen. Collagenase activity was found in each specimen. Lungs of 10 untreated dogs served as controls. The collagenolytic system of the transplanted lungs was inhibited by EDTA only. No inhibitory effect was obtained by the inhibitor of bacterial collagenase, cysteine, and normal serum containing protease inhibitors. By means of inhibition studies it was tried to determine the origin of the collagenolytic activity. It is suggested that the enzyme effect is derived from the lysosomes of polymorphonuclear leukocytes. Collagenase of granulocytic origin seems to participate as mediator in the immunological reaction of hyperacute lung graft rejection.
6只杂种犬经全层皮肤移植致敏，随后进行原位肺同种异体移植。将超急性排斥的肺移植组织外植体置于天然胶原凝胶表面。在每个标本中均发现了胶原酶活性。10只未处理犬的肺作为对照。移植肺的胶原溶解系统仅被EDTA抑制。细菌胶原酶抑制剂、半胱氨酸和含蛋白酶抑制剂的正常血清均未产生抑制作用。通过抑制研究试图确定胶原溶解活性的来源。提示该酶作用源自多形核白细胞的溶酶体。粒细胞来源的胶原酶似乎作为介质参与了超急性肺移植排斥的免疫反应。
