Laboratoire de Microbiologie IRD, UMR 180, Aix-Marseille Université, Marseille, France.
Syst Appl Microbiol. 2011 Dec;34(8):581-5. doi: 10.1016/j.syapm.2011.04.001. Epub 2011 May 18.
Cultivated members of the order Thermotogales comprise only thermophilic to hyperthermophilic anaerobic microorganisms. However, based on molecular studies, the existence of mesophilic members ("mesotoga") within this order has been postulated but has not been demonstrated by cultural approaches so far. A "mesotoga" (strain PhosAc3) that belonged to an uncultivated lineage distantly related to the thermophilic Kosmotoga genus has now been cultivated in axenic culture. It grew between 30°C and 50°C (optimum 40°C) and oxidized lactate using elemental sulphur as a terminal electron acceptor. Further genomic and physiological characterization of strain PhosAc3 will be important not only for understanding bacterial adaptation to high and moderate temperatures at small evolutionary scales, but also because "mesotoga" might play a crucial ecological role in ecosystems polluted by aromatic compounds.
热脱硫杆菌目中的培养成员仅包括嗜热至超嗜热的厌氧微生物。然而，基于分子研究，已经推测出该目中存在中温成员（“mesotoga”），但迄今为止尚未通过培养方法证明。现在已经在无菌培养中培养了一种属于与嗜热 Kosmotoga 属亲缘关系较远的未培养谱系的“mesotoga”（菌株 PhosAc3）。它在 30°C 和 50°C 之间生长（最佳温度为 40°C），并使用元素硫作为末端电子受体氧化乳酸盐。对 PhosAc3 菌株进行进一步的基因组和生理学特性分析不仅对于理解细菌在小进化尺度上对高温和中温的适应非常重要，而且因为“mesotoga”可能在受芳香族化合物污染的生态系统中发挥关键的生态作用。
