文章：HIV-1 进入辅助因子：七跨膜、G 蛋白偶联受体的功能性 cDNA 克隆。科学。1996. 272: 872-877.
Pillars article: HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996. 272: 872-877.
出版信息
J Immunol. 2011 Jun 1;186(11):6076-81.
Abstract
A cofactor for HIV-1 (human immunodeficiency virus-type 1) fusion and entry was identified with the use of a novel functional complementary DNA (cDNA) cloning strategy. This protein, designated “fusin,” is a putative G protein–coupled receptor with seven transmembrane segments. Recombinant fusin enabled CD4-expressing nonhuman cell types to support HIV-1 Env-mediated cell fusion and HIV-1 infection. Antibodies to fusin blocked cell fusion and infection with normal CD4-positive human target cells. Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line–tropic isolates, in comparison to its activity with macrophage-tropic HIV-1 isolates.
摘要
利用一种新颖的功能性 cDNA 克隆策略，鉴定出 HIV-1（人类免疫缺陷病毒-1 型）融合和进入的辅助因子。这种名为“融合蛋白”的蛋白是一种假定的 G 蛋白偶联受体，具有七个跨膜片段。重组融合蛋白使表达 CD4 的非人类细胞类型能够支持 HIV-1 Env 介导的细胞融合和 HIV-1 感染。针对融合蛋白的抗体可阻断细胞融合和正常 CD4 阳性人类靶细胞的感染。融合蛋白信使 RNA 水平与不同人类细胞类型中 HIV-1 的允许性相关。与巨噬细胞嗜性 HIV-1 分离株相比，融合蛋白优先作用于 T 细胞系嗜性分离株。
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