卵泡液中基质细胞衍生因子-1（SDF-1/CXCL12）的浓度与囊胚发育相关。
Concentration of stromal cell-derived factor-1 (SDF-1/CXCL12) in the follicular fluid is associated with blastocyst development.
作者信息
Nishigaki Akemi, Tsuzuki-Nakao Tomoko, Kido Takeharu, Kida Naoko, Kakita-Kobayashi Maiko, Murata Hiromi, Okada Hidetaka
机构信息
Department of Obstetrics and Gynecology Kansai Medical University Hirakata Osaka Japan.
出版信息
Reprod Med Biol. 2018 Nov 28;18(2):161-166. doi: 10.1002/rmb2.12259. eCollection 2019 Apr.
PURPOSE
To study the association between stromal cell-derived factor-1 (SDF-1/CXCL12) and vascular endothelial growth factor (VEGF) concentrations in individual human ovarian follicles and IVF outcomes.
METHODS
Concentrations of SDF-1 and VEGF in 261 follicular fluid samples were measured with enzyme-linked immunosorbent assay. IVF outcome parameters were included in fertilization rate, cleavage rate, embryo morphology on day 3, and blastocyst morphology on day 5.
RESULTS
The follicular concentration of SDF-1 and VEGF was not significantly associated with fertilization and cleavage outcome, and embryo morphology. The rates of full blastocysts and good-quality blastocysts were significantly higher in follicles with an SDF-1 concentration of 275-350 pg/mL than in the follicles with SDF-1 concentrations of <200 and ≥350 pg/mL (0.05). The follicular concentration of VEGF was not associated with the blastocyst morphology.
CONCLUSION
Our findings showed that follicular concentration of SDF-1, and not VEGF, may be a valuable biochemical marker of blastocyst development.
目的
研究人单个卵巢卵泡中基质细胞衍生因子-1（SDF-1/CXCL12）和血管内皮生长因子（VEGF）浓度与体外受精结局之间的关联。
方法
采用酶联免疫吸附测定法测量261份卵泡液样本中SDF-1和VEGF的浓度。体外受精结局参数包括受精率、卵裂率、第3天的胚胎形态和第5天的囊胚形态。
结果
卵泡中SDF-1和VEGF的浓度与受精和卵裂结局以及胚胎形态无显著关联。SDF-1浓度为275 - 350 pg/mL的卵泡中完全囊胚和优质囊胚的发生率显著高于SDF-1浓度<200 pg/mL和≥350 pg/mL的卵泡（P<0.05）。卵泡中VEGF的浓度与囊胚形态无关。
结论
我们的研究结果表明，卵泡中SDF-1的浓度而非VEGF的浓度可能是囊胚发育的一个有价值的生化标志物。
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