Bolin S R, Ridpath J F
USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA 50010.
Am J Vet Res. 1990 May;51(5):703-7.
The range of neutralizing activity to bovine viral diarrhea (BVD) virus and viral protein specificity of antibodies induced by 3 inactivated vaccines were evaluated by use of samples of sera obtained from 13 cattle 14 days after vaccination. Viral neutralizing antibodies wee detected in all cattle to each of 10 noncytopathic and 10 cytopathic isolates of BVD virus. A viral-induced polypeptide (53,000 to 56,000 daltons) was detected by radioimmunoprecipitation with serum from all vaccinates. Other viral-induced polypeptides of 115,000, 80,000, 48,000, and 25,000 daltons were precipitated with sera from some vaccinates. Precipitation of those polypeptides was related to the vaccine used. When multiple viral polypeptides were precipitated, the 53,000- to 56,000-dalton polypeptide appeared immunodominant.
利用接种疫苗14天后从13头牛采集的血清样本，评估了3种灭活疫苗诱导产生的抗体对牛病毒性腹泻（BVD）病毒的中和活性范围及病毒蛋白特异性。在所有牛中均检测到针对10种非致细胞病变型和10种致细胞病变型BVD病毒分离株的病毒中和抗体。通过对所有接种疫苗动物的血清进行放射免疫沉淀，检测到一种病毒诱导的多肽（53,000至56,000道尔顿）。其他115,000、80,000、48,000和25,000道尔顿的病毒诱导多肽在部分接种疫苗动物的血清中出现沉淀。这些多肽的沉淀与所用疫苗有关。当多种病毒多肽出现沉淀时，53,000至56,000道尔顿的多肽表现为免疫显性。
