Department of Biomedical Sciences and Technologies, Section of Radiological Sciences, University of Milan, via di Rudinì 8, 20142 Milan, Italy.
Mol Imaging Biol. 2012 Apr;14(2):183-96. doi: 10.1007/s11307-011-0496-0.
The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model.
Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or (111)In-oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV-Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data.
DC labeling, both with MNPs and with (111)In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and (111)In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs.
MRI and SPECT imaging, by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.
作者提出了一种方案，使用不同的成像技术，在一种鼠乳腺癌模型中评估肿瘤特异性树突状细胞（DC）向淋巴结（LN）归巢的体内情况。
骨髓 DC 用顺磁纳米粒子（MNPs）或（111）In-oxine 标记。使用肿瘤裂解物进行抗原负载。成熟的 DC 被注射到转基因荷瘤小鼠（MMTV-Ras）的足垫中，并通过磁共振成像（MRI）和单光子发射计算机断层扫描（SPECT）跟踪 DC 迁移。进行了离体分析以验证成像数据。
MNPs 和（111）In-oxine 标记的 DC 对 DC 表型或功能没有影响。MRI 和 SPECT 允许在腋窝和腘窝 LN 中检测到铁和（111）In。免疫组织化学和γ计数显示 LN 中存在 DC。
MRI 和 SPECT 成像通过允许体内动态监测 DC 迁移，可进一步开发和优化有效的抗癌疫苗。
