Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institute of Health (NIH), Bethesda, MD 20892, USA.
Nanoscale. 2011 Jan;3(1):142-53. doi: 10.1039/c0nr00493f. Epub 2010 Oct 11.
Cell based therapeutics are emerging as powerful regimens. To better understand the migration and proliferation mechanisms of implanted cells, a means to track cells in living subjects is essential, and to achieve that, a number of cell labeling techniques have been developed. Nanoparticles, with their superior physical properties, have become the materials of choice in many investigations along this line. Owing to inherent magnetic, optical or acoustic attributes, these nanoparticles can be detected by corresponding imaging modalities in living subjects at a high spatial and temporal resolution. These features allow implanted cells to be separated from host cells; and have advantages over traditional histological methods, as they permit non-invasive, real-time tracking in vivo. This review attempts to give a summary of progress in using nanotechnology to monitor cell trafficking. We will focus on direct cell labeling techniques, in which cells ingest nanoparticles that bear traceable signals, such as iron oxide or quantum dots. Ferritin and MagA reporter genes that can package endogenous iron or iron supplement into iron oxide nanoparticles will also be discussed.
基于细胞的治疗方法正在兴起，成为一种强大的治疗方案。为了更好地了解植入细胞的迁移和增殖机制，需要一种能够在活体中追踪细胞的方法，为此，已经开发了许多细胞标记技术。由于具有优越的物理性质，纳米粒子已成为许多此类研究中首选的材料。由于具有固有磁性、光学或声学属性，这些纳米粒子可以通过相应的成像模式在活体中以高空间和时间分辨率进行检测。这些特征使得可以将植入的细胞与宿主细胞分离；并且与传统的组织学方法相比具有优势，因为它们允许在体内进行非侵入性、实时跟踪。本综述试图总结使用纳米技术监测细胞迁移的进展。我们将重点介绍直接细胞标记技术，其中细胞摄取带有可追踪信号的纳米粒子，例如氧化铁或量子点。还将讨论可以将内源性铁或铁补充剂包装到氧化铁纳米粒子中的 Ferritin 和 MagA 报告基因。
