Medical Research Center, The Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, People's Republic of China.
Mol Biol Rep. 2012 Feb;39(2):1267-74. doi: 10.1007/s11033-011-0859-2. Epub 2011 May 21.
Annexin A2 and Cdc42 were identified by 2-dimensional electrophoresis (2-DE) and MALDI-TOF-MS between esophageal squamous cell carcinomas (ESCC) and corresponding normal esophagus mucosa in our previous study. To assess clinico-pathological pattern and Annexin A2 and Cdc42 status with respect to cell differentiation and lymphnode metastasis in patients with ESCC. The expression of Annexin A2 and Cdc42 in 22 pairs of fresh ESCC and matched tissues were detected by qRT-PCR and western blot, respectively. And it was further confirmed by immunohistochemistry with 175 pairs of formalin-fixed, paraffin-embedded ESCC. Results showed that Annexin A2 expression was significantly down-regulated, and Cdc42 was up-regulated in ESCC compared to matched control on both mRNA and protein level (P < 0.05), which was in accordance with our previous results on proteomics data. Additionally, Annexin A2 and Cdc42 expression was significantly correlated with lymphoid node metastasis (P < 0.05) and pathological differentiation (P < 0.05). Taken together, we proposed that the aberrant expression of Annexin A2 and Cdc42 played a role in carcinogenesis, differentiation and metastasis of ESCC, which implied its potential target for clinical biomarkers in differentiation and lymph node metastasis.
在我们之前的研究中，通过二维电泳（2-DE）和 MALDI-TOF-MS 在食管鳞状细胞癌（ESCC）和相应的正常食管黏膜之间鉴定出 Annexin A2 和 Cdc42。为了评估 Annexin A2 和 Cdc42 的临床病理模式以及 ESCC 患者的细胞分化和淋巴结转移状态。通过 qRT-PCR 和 Western blot 分别检测了 22 对新鲜 ESCC 和匹配组织中 Annexin A2 和 Cdc42 的表达，并通过 175 对福尔马林固定、石蜡包埋的 ESCC 进行了免疫组织化学进一步证实。结果表明，与匹配对照相比， Annexin A2 的表达在 mRNA 和蛋白水平上均显著下调，而 Cdc42 则上调（P <0.05），这与我们之前的蛋白质组学数据结果一致。此外，Annexin A2 和 Cdc42 的表达与淋巴结转移（P <0.05）和病理分化（P <0.05）显著相关。综上所述，我们提出 Annexin A2 和 Cdc42 的异常表达在 ESCC 的发生、分化和转移中起作用，这意味着其在分化和淋巴结转移中作为临床生物标志物具有潜在的靶向作用。
