Zhou Zhenhua, Li Yan, Xu Leqin, Wang Xudong, Chen Su, Yang Cheng, Xiao Jianru
Department of Bone Tumor Surgery, Changzheng Hospital, The Second Military Medical University, No.415, Fengyang Road, Shanghai, 200433, China.
Department of Orthopaedics, The 169th Hospital of PLA, Hengyang, Hunan, China.
Tumour Biol. 2016 Jul;37(7):9681-9. doi: 10.1007/s13277-016-4867-7. Epub 2016 Jan 23.
Giant cell tumor of bone(GCTB) is a special bone tumor for it consists of various cell types, and its biological characteristics is different from common benign or malignant neoplasm. In the present study, we report the biological features of a primary Asian GCTB cell line named GCTB28. We analyzed extensive properties of the GCTB28 cells including morphological observations, growth, cell cycle, karyotype, proliferation, proteins expression, surface biomarker verification, and tumorigenicity in nude mice. We found that the stromal cells of GCTB were endowed with self-renewal capacity and played dominant roles in GCTB development. Moreover, we confirmed that GCTB cells can be CD33(-)CD14(-) phenotype which was not in accord with previous study. This study provides an in vitro model system to investigate pathogenic mechanisms and molecular characteristics of GCTB and also provides a useful tool for researching the therapeutic targeting of GCTB.
骨巨细胞瘤（GCTB）是一种特殊的骨肿瘤，因为它由多种细胞类型组成，其生物学特性不同于常见的良性或恶性肿瘤。在本研究中，我们报告了一种名为GCTB28的原发性亚洲GCTB细胞系的生物学特征。我们分析了GCTB28细胞的广泛特性，包括形态学观察、生长、细胞周期、核型、增殖、蛋白质表达、表面生物标志物验证以及在裸鼠中的致瘤性。我们发现GCTB的基质细胞具有自我更新能力，并在GCTB的发展中起主导作用。此外，我们证实GCTB细胞可以是CD33(-)CD14(-)表型，这与先前的研究不一致。本研究提供了一个体外模型系统来研究GCTB的致病机制和分子特征，也为研究GCTB的治疗靶点提供了一个有用的工具。
