Laboratory of Clinical Chemistry and Hematology, Academic Hospital of Parma, Parma, Italy.
Laboratory of Clinical Biochemistry, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.
Clin Chim Acta. 2014 Sep 25;436:183-7. doi: 10.1016/j.cca.2014.05.028. Epub 2014 Jun 8.
This study was planned to establish whether random orientation of gel tubes after centrifugation may impair sample quality.
Eight gel tubes were collected from 17 volunteers: 2 Becton Dickinson (BD) serum tubes, 2 Terumo serum tubes, 2 BD lithium heparin tubes and 2 Terumo lithium heparin tubes. One patient's tube for each category was kept in a vertical, closure-up position for 90 min ("upright"), whereas paired tubes underwent bottom-up inversion every 15 min, for 90 min ("inverted"). Immediately after this period of time, 14 clinical chemistry analytes, serum indices and complete blood count were then assessed in all tubes.
Significant increases were found for phosphate and lipaemic index in all inverted tubes, along with AST, calcium, cholesterol, LDH, potassium, hemolysis index, leukocytes, erythrocytes and platelets limited to lithium heparin tubes. The desirable quality specifications were exceeded for AST, LDH, and potassium in inverted lithium heparin tubes. Residual leukocytes, erythrocytes, platelets and cellular debris were also significantly increased in inverted lithium heparin tubes.
Lithium heparin gel tubes should be maintained in a vertical, closure-up position after centrifugation.
本研究旨在确定离心后凝胶管的随机方向是否会影响样品质量。
从 17 名志愿者中收集了 8 个凝胶管：2 个 BD 血清管、2 个 Terumo 血清管、2 个 BD 锂肝素管和 2 个 Terumo 锂肝素管。每种类别的一个患者管保持垂直、封闭状态 90 分钟（“直立”），而配对管每隔 15 分钟进行一次底部向上翻转，持续 90 分钟（“倒置”）。在此时间段后，立即评估所有管中的 14 项临床化学分析物、血清指数和全血细胞计数。
所有倒置管中的磷酸盐和脂血症指数均显著升高，同时 AST、钙、胆固醇、LDH、钾、溶血指数、白细胞、红细胞和血小板仅限于锂肝素管。倒置锂肝素管中的 AST、LDH 和钾超过了理想的质量规格。倒置锂肝素管中的残留白细胞、红细胞、血小板和细胞碎片也显著增加。
离心后，锂肝素凝胶管应保持垂直、封闭状态。
