Department of Periodontology, Dental School, University of Bern, Freiburgstrasse 7, CH-3010 Bern, Switzerland.
Clin Oral Investig. 2012 Jun;16(3):735-44. doi: 10.1007/s00784-011-0567-2. Epub 2011 May 24.
The antimicrobial effect of taurolidine was tested against periodontopathic species in comparison to chlorhexidine digluconate in the presence or absence of serum. Minimal inhibitory concentrations (MIC), microbiocidal concentrations (MBC), as well as killing were determined against 32 different microbial strains including 3 Porphyromonas gingivalis, 3 Aggregatibacter actinomycetemcomitans, and 15 potentially superinfecting species with and without 25% v/v human serum. The MIC(50) of taurolidine against the tested microbial strains was 0.025% and the MIC(90) 0.05%. The respective values for the MBCs were 0.05% and 0.1%. Addition of 25% serum (heat-inactivated) did not change the MIC and MBC values of taurolidine. In contrast, MICs and MBCs of chlorhexidine (CHX) increased by two steps after addition of serum. Taurolidine killed microorganisms in a concentration and time-dependent manner, the killing rate of 1.6% taurolidine was 99.08% ± 2.27% in mean after 2 h. Again, killing activity of taurolidine was not affected if serum was added, whereas addition of inactivated serum clearly reduced the killing rate of all selected bacterial strains by CHX. Therefore, taurolidine possesses antimicrobial properties which are not reduced in the presence of serum as a main component in gingival crevicular fluid and wound fluid. Taurolidine may have potential as an antimicrobial agent in non-surgical and surgical periodontal treatment.
牛磺罗定的抗菌作用在有或没有血清的情况下，与洗必泰葡萄糖酸盐进行了比较，以测试其对牙周病相关种的抑制作用。针对 32 种不同的微生物菌株（包括 3 种牙龈卟啉单胞菌、3 种伴放线放线杆菌和 15 种可能的超感染种），测定了最小抑菌浓度（MIC）、杀菌浓度（MBC）和杀菌作用。牛磺罗定对测试的微生物菌株的 MIC(50)为 0.025%，MIC(90)为 0.05%。相应的 MBC 值分别为 0.05%和 0.1%。添加 25%（热灭活）血清不会改变牛磺罗定的 MIC 和 MBC 值。相比之下，添加血清后，洗必泰（CHX）的 MIC 和 MBC 值增加了两步。牛磺罗定以浓度和时间依赖的方式杀死微生物，在 2 小时后，1.6%牛磺罗定的杀菌率平均值为 99.08%±2.27%。同样，如果添加血清，牛磺罗定的杀菌活性不会受到影响，而添加热灭活血清会明显降低 CHX 对所有选定细菌菌株的杀菌率。因此，牛磺罗定具有抗菌特性，在龈沟液和伤口液的主要成分血清存在的情况下，其抗菌特性不会降低。牛磺罗定可能具有作为非手术和手术牙周治疗的抗菌剂的潜力。
