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漱口水对体外培养的口腔生物膜组成及代谢活性的影响。

Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro.

作者信息

Fernandez Y Mostajo Mercedes, Exterkate Rob A M, Buijs Mark J, Crielaard Wim, Zaura Egija

机构信息

Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Free University Amsterdam, Gustav Mahlerlaan 3004, 1081, LA, Amsterdam, The Netherlands.

出版信息

Clin Oral Investig. 2017 May;21(4):1221-1230. doi: 10.1007/s00784-016-1876-2. Epub 2016 Jun 23.

DOI:10.1007/s00784-016-1876-2
PMID:27337976
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5399055/
Abstract

OBJECTIVE

The aim of this study was to determine the effect of an oxygenating mouthwash compared to two other established mouthwash products on bacterial composition and metabolic activity of oral biofilms in vitro.

MATERIAL AND METHODS

Twelve healthy subjects participated as donors. Plaque-saliva mixture inoculated biofilms were grown and treated with 3 different chemotherapeutic mouthwashes [amine fluoride/stannous fluoride (MD), oxygenating agent (AX), chlorhexidine 0.12 % (PA), and water (W)]. Effects of treatments were assessed on biofilm composition (16S rRNA gene amplicon sequencing), production of organic acids (formate, acetate, lactate, propionate, butyrate using capillary electrophoresis), and viability of the remaining biofilm (CFUs).

RESULTS

Microbial profiles of biofilms clustered per inoculum donor and were dominated by the genera Veillonella, Streptococcus, and Prevotella. Microbial diversity was only reduced after PA treatment. Significant changes in composition occurred after treatment with AX, resulting in lower proportions of Veillonella and higher proportions of non-mutans streptococci. Production of all organic acids after PA and lactate after MD was significantly lower as compared to W. AX resulted in reduction of acetate, butyrate, and propionate and increase in lactate production (p < 0.05). Viable counts were significantly lower after PA and AX treatments compared to W, while no significant reduction was observed after MD.

CONCLUSIONS

All studied mouthwashes affected the in vitro biofilms differently. The effects of the AX treatment were the most prominent which resulted in changes of the bacterial composition and metabolism.

CLINICAL IMPLICATIONS

Awareness by the dental team that mouthwashes can change the bacterial composition and metabolism is important when advising its use.

摘要

目的

本研究旨在确定一种含氧化成分的漱口水与其他两种已确立的漱口水产品相比，对体外口腔生物膜细菌组成和代谢活性的影响。

材料与方法

12名健康受试者作为供体参与研究。接种菌斑 - 唾液混合物的生物膜生长后，用3种不同的化学治疗漱口水进行处理[胺氟化物/氟化亚锡（MD）、氧化成分（AX）、0.12%氯己定（PA）和水（W）]。通过生物膜组成（16S rRNA基因扩增子测序）、有机酸（甲酸、乙酸、乳酸、丙酸、丁酸，采用毛细管电泳法检测）产生情况以及剩余生物膜的活力（菌落形成单位）来评估处理效果。

结果

生物膜的微生物谱根据接种供体聚类，主要由韦荣氏菌属、链球菌属和普雷沃氏菌属主导。仅在PA处理后微生物多样性降低。AX处理后生物膜组成发生显著变化，导致韦荣氏菌比例降低，非变形链球菌比例升高。与W相比，PA处理后所有有机酸的产生以及MD处理后乳酸的产生均显著降低。AX导致乙酸、丁酸和丙酸减少，乳酸产生增加（p < 0.05）。与W相比，PA和AX处理后活菌数显著降低，而MD处理后未观察到显著减少。

结论

所有研究的漱口水对体外生物膜的影响各不相同。AX处理的效果最为显著，导致细菌组成和代谢发生变化。

临床意义

牙科团队应意识到漱口水可改变细菌组成和代谢，这在建议使用漱口水时很重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/11499e0623ca/784_2016_1876_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/8b59c801e185/784_2016_1876_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/3d9724f37af2/784_2016_1876_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/fe37805a0283/784_2016_1876_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/05cc5207e621/784_2016_1876_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/11499e0623ca/784_2016_1876_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/8b59c801e185/784_2016_1876_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/3d9724f37af2/784_2016_1876_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/fe37805a0283/784_2016_1876_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/05cc5207e621/784_2016_1876_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/5399055/11499e0623ca/784_2016_1876_Fig5_HTML.jpg

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