Zhou Wei, Li Yong-jin, Wei Pin-kang
Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Apr;31(4):532-6.
To study the effect of Xiaotan Sanjie Recipe (XTSJD) and its mechanism on vasculogenic mimicry (VM) of human gastric cancer xenografts in nude mice.
The tumor-bearing mice model was established by subcutaneous inoculating with xenografts of human gastric cancer into the right armpit of 30 BALB/c nude mice. After modeling, the tumor-bearing mice were randomly divided into the normal saline group, the XTSJD group, and the doxycycline hyclate (DH) group, 10 in each. And the mice were administered with corresponding medicine by gastrogavage for 4 weeks. Then all mice were killed by cervical dislocation. The tumor mass were weighed and the tumor inhibition rate calculated. The amount of VM in tumor was counted. Expressions of matrix metalloproteinase (MMP) -2 and MMP-9 were tested using immunohistochemical method.
Tumor weight in the XTSJD group and the OH group decreased significantly when compared with the NS group (P<0.01). The amount of VM in the XTSJD group (24.50+/-3.03) and the OH group (14.70+/-1.34) was significantly less than that in the NS group (33.10+/-2.64) (P<0.01). The positive expressions of MMP-2 and MMP-9 in the XTSJD group and the OH group was significantly lower than that in the NS group (P <0.01).
XTSJD could inhibit the formation of VM in xenografted tumor of nude mice. The mechanism might be correlated with the down-regulation of MMP-2 and MMP-9 expressions.
研究消痰散结方（XTSJD）对人胃癌裸鼠移植瘤血管生成拟态（VM）的影响及其机制。
将30只BALB/c裸鼠右腋窝皮下接种人胃癌移植瘤建立荷瘤小鼠模型。造模后，将荷瘤小鼠随机分为生理盐水组、消痰散结方组和盐酸多西环素（DH）组，每组10只。通过灌胃给予小鼠相应药物，持续4周。然后通过颈椎脱臼法处死所有小鼠。称取肿瘤质量并计算肿瘤抑制率。计数肿瘤中VM的数量。采用免疫组织化学方法检测基质金属蛋白酶（MMP）-2和MMP-9的表达。
与生理盐水组相比，消痰散结方组和DH组的肿瘤重量显著降低（P<0.01）。消痰散结方组（24.50±3.03）和DH组（14.70±1.34）的VM数量显著少于生理盐水组（33.10±2.64）（P<0.01）。消痰散结方组和DH组中MMP-2和MMP-9的阳性表达显著低于生理盐水组（P<0.01）。
消痰散结方可抑制裸鼠移植瘤中VM的形成。其机制可能与下调MMP-2和MMP-9的表达有关。
