Ye Min, Sun Da-Zhi, Wei Pin-kang
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 May;34(5):592-6.
To study the inhibitory effect of Xiaotan Sanjie Recipe (XSR) on the microsatellite instability of orthotopic transplantation tumor in MKN-45 human gastric cancer nude mice.
The 3rd passage subcutaneous transplantation tumor was taken as the origin of the model by using MKN-45 human gastric cancer cell lines. MKN-45 human gastric cancer nude mouse model was established using OB glue adhesive method. Then 30 nude mice were divided into the model group, the XSR group, and the chemotherapy group. Mice in the XSR group were intragastrically given XSR at the daily dose of 0.4 mL. Mice in the chemotherapy group were intragastrically given Fluorouracil at the daily dose of 0.4 mL. No intervention was given to mice in the model group. After 6 weeks of medication, the tumor weight was measured, and the tumor inhibition rate calculated. The size, the peak height, and the peak area of 5 microsatellite instability sites were detected.
The tumor inhibition rate was 40. 84% in the XSR group. The tumor weight was significantly lower in the XSR group than in the model group (P < 0.01), showing no statistical difference when compared with the chemotherapy group (P >0.05). The incidence of high microsatellite instability (MSI-H) in the model group was 70%, and the incidence of low microsatellite instability (MSI-L) was 30%. Microsatellite stable site tended be stable after 6 weeks of XSR treatment.
XSR showed inhibition on microsatellite instable orthotopic transplantation tumor in MKN-45 human gastric cancer nude mice.
研究消痰散结方（XSR）对MKN-45人胃癌裸鼠原位移植瘤微卫星不稳定性的抑制作用。
以第3代皮下移植瘤为模型来源，采用MKN-45人胃癌细胞系，用OB胶粘贴法建立MKN-45人胃癌裸鼠模型。将30只裸鼠分为模型组、XSR组和化疗组。XSR组小鼠每日灌胃给予XSR，剂量为0.4 mL。化疗组小鼠每日灌胃给予氟尿嘧啶，剂量为0.4 mL。模型组小鼠不给予干预。给药6周后，测量肿瘤重量并计算肿瘤抑制率。检测5个微卫星不稳定性位点的大小、峰高和峰面积。
XSR组肿瘤抑制率为40.84%。XSR组肿瘤重量明显低于模型组（P<0.01），与化疗组比较无统计学差异（P>0.05）。模型组高微卫星不稳定性（MSI-H）发生率为70%，低微卫星不稳定性（MSI-L）发生率为30%。XSR治疗6周后微卫星稳定位点趋于稳定。
XSR对MKN-45人胃癌裸鼠微卫星不稳定原位移植瘤具有抑制作用。
