Tam Charmaine S, Heilbronn Leonie K, Henegar Corneliu, Wong Melanie, Cowell Christopher T, Cowley Mark J, Kaplan Warren, Clément Karine, Baur Louise A
Institute of Endocrinology and Diabetes, The Children's Hospital, University of Sydney , Westmead , Australia .
Int J Pediatr Obes. 2011 Jun;6(2-2):e360-3. doi: 10.3109/17477166.2011.575152. Epub 2011 May 24.
The aim of this study was to characterize expression profiles of visceral and subcutaneous adipose tissue in children. Adipose tissue samples were collected from children having elective surgery (n = 71, [54 boys], 6.0 ± 4.3 years). Affymetrix microarrays (n = 20) were performed to characterize the functional profile and identify genes of interest in adipose tissue. Visceral adipose tissue had an overrepresentation of Gene Ontology themes related to immune and inflammatory responses and subcutaneous adipose tissue had an overrepresentation of themes related to adipocyte growth and development. Likewise, qPCR performed in the whole cohort showed a 30-fold increase in haptoglobin (P = 0.005), 7-fold increase in IL-10 (P < 0.001), 8-fold decrease in VEGF (P = 0.01) and a 28-fold decrease in TBOX15 (P < 0.001) in visceral compared to subcutaneous adipose tissue. The inflammatory pattern in visceral adipose tissue may represent an early stage of the adverse effects of this depot, and combined with chronic obesity, may contribute to increased metabolic and cardiovascular risk.
本研究的目的是描述儿童内脏和皮下脂肪组织的表达谱。从接受择期手术的儿童（n = 71，[54名男孩]，6.0±4.3岁）中收集脂肪组织样本。使用Affymetrix微阵列（n = 20）来描述功能谱并鉴定脂肪组织中感兴趣的基因。内脏脂肪组织中与免疫和炎症反应相关的基因本体主题过度表达，而皮下脂肪组织中与脂肪细胞生长和发育相关的主题过度表达。同样，在整个队列中进行的qPCR显示，与皮下脂肪组织相比，内脏脂肪组织中的触珠蛋白增加了30倍（P = 0.005），IL-10增加了7倍（P <0.001），VEGF减少了8倍（P = 0.01），TBOX15减少了28倍（P <0.001）。内脏脂肪组织中的炎症模式可能代表了该脂肪库不良反应的早期阶段，并且与慢性肥胖相结合，可能会增加代谢和心血管风险。
