Roberton A M, Harris P J, Hollands H J, Ferguson L R
Department of Biochemistry, University of Auckland, New Zealand.
Mutat Res. 1990 Jun;244(2):173-8. doi: 10.1016/0165-7992(90)90068-u.
The adsorption of 1,8-dinitropyrene (DNP) to alpha-cellulose has been studied as a model system for examining the adsorption of a hydrophobic mutagen to dietary fiber. Most of the DNP rapidly disappeared from an aqueous solution and partitioned between the glass wall of the test tube and the alpha-cellulose. Factors affecting DNP distribution included (i) the time of incubation, (ii) the final concentration of the solvent, dimethyl sulphoxide, in which the DNP has been dissolved, and (iii) the relative concentrations of DNP and alpha-cellulose. We suggest that this model system could be applied to other mutagens, and that alpha-cellulose would provide a useful standard fiber to permit inter-laboratory comparisons.
作为研究疏水性诱变剂在膳食纤维上吸附情况的模型系统，已对1,8 -二硝基芘（DNP）在α-纤维素上的吸附进行了研究。大部分DNP迅速从水溶液中消失，并在试管玻璃壁和α-纤维素之间分配。影响DNP分布的因素包括：（i）孵育时间；（ii）溶解DNP的溶剂二甲基亚砜的最终浓度；（iii）DNP和α-纤维素的相对浓度。我们认为该模型系统可应用于其他诱变剂，并且α-纤维素将提供一种有用的标准纤维，以便进行实验室间比较。
