Department of Conservative and Preventive Dentistry, Dental Clinic, Justus Liebig University, Giessen, Germany.
Caries Res. 2011;45 Suppl 1:13-23. doi: 10.1159/000326819. Epub 2011 May 31.
The advantages, limitations and potential applications of available methods for studying erosion of enamel and dentine are reviewed. Special emphasis is placed on the influence of histological differences between the dental hard tissue and the stage of the erosive lesion. No method is suitable for all stages of the lesion. Factors determining the applicability of the methods are: surface condition of the specimen, type of the experimental model, nature of the lesion, need for longitudinal measurements and type of outcome. The most suitable and most widely used methods are: chemical analyses of mineral release and enamel surface hardness for early erosion, and surface profilometry and microradiography for advanced erosion. Morphological changes in eroded dental tissue have usually been characterised by scanning electron microscopy. Novel methods have also been used, but little is known of their potential and limitations. Therefore, there is a need for their further development, evaluation, consolidation and, in particular, validation.
本文回顾了现有的研究牙釉质和牙本质侵蚀的方法的优缺点和潜在应用。特别强调了牙体硬组织的组织学差异和侵蚀病变阶段的影响。没有一种方法适用于病变的所有阶段。决定方法适用性的因素有：标本的表面状况、实验模型的类型、病变的性质、纵向测量的需要和结果的类型。最适用和最广泛使用的方法是：对早期侵蚀进行矿物释放和牙釉质表面硬度的化学分析，对晚期侵蚀进行表面轮廓测量和显微射线照相。侵蚀牙体组织的形态变化通常通过扫描电子显微镜来描述。也使用了一些新方法，但对它们的潜力和局限性知之甚少。因此，需要进一步开发、评估、整合，特别是验证这些方法。
