Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
Lab Chip. 2011 Jul 21;11(14):2333-42. doi: 10.1039/c1lc20147f. Epub 2011 Jun 1.
We present a dielectrophoresis (DEP)-based cellular microarray chip for cell-based anticancer drug screening in perfusion microenvironments. Human breast cancer cells, MCF7, were seeded into the chip and patterned via DEP forces onto the planar interdigitated ring electrode (PIRE) arrays. Roughly, only one third of the cell amount was required for the chip compared to that for a 96-well plate control. Drug concentrations (cisplatin or docetaxel) were stably generated by functional integration of a concentration gradient generator (CGG) and an anti-crosstalk valve (ACV) to treat cells for 24 hours. Cell viability was quantified using a dual staining method. Results of cell patterning show substantial uniformity of patterned cells (92 ± 5 cells per PIRE). Furthermore, after 24 hour drug perfusion, no statistical significance in dose-responses between the chip and the 96-well plate controls was found. The IC(50) value from the chip also concurred with the values from the literature. Moreover, the perfusion culture exhibited reproducibility of drug responses of cells on different PIREs in the same chamber. The chip would enable applications where cells are of limited supply, and supplement microfluidic perfusion cultures for clinical practices.
我们提出了一种基于介电泳（DEP）的细胞微阵列芯片，用于在灌注微环境中进行基于细胞的抗癌药物筛选。将人乳腺癌细胞 MCF7 通过 DEP 力接种到芯片上，并在平面叉指环电极（PIRE）阵列上进行图案化。与 96 孔板对照相比，该芯片所需的细胞数量大约减少了三分之一。通过浓度梯度发生器（CGG）和抗串扰阀（ACV）的功能集成，稳定地产生药物浓度（顺铂或多西他赛），以处理细胞 24 小时。使用双重染色法定量细胞活力。细胞图案化的结果显示出图案化细胞的高度均匀性（每个 PIRE 有 92 ± 5 个细胞）。此外，在 24 小时药物灌注后，芯片与 96 孔板对照之间的剂量反应没有统计学意义。芯片的 IC50 值也与文献值一致。此外，在相同腔室内，灌注培养显示出细胞对不同 PIRE 的药物反应的重现性。该芯片将能够应用于细胞供应有限的情况，并补充微流控灌注培养用于临床实践。
