Institute for Systems Research (ISR), University of Maryland, College Park, MD 20742, USA.
Lab Chip. 2011 Jul 21;11(14):2316-8. doi: 10.1039/c1lc20306a. Epub 2011 Jun 1.
Programmable 3D cell assembly under physiological pH conditions is achieved using electrodeposited stimuli-responsive alginate gels in a microfluidic device, with parallel sidewall electrodes enabling direct observation of the cell assembly. Electrically triggered assembly and subsequent viability of mammalian cells is demonstrated, along with spatially programmable, multi-address assembly of different strains of E. coli cells. Our approach enables in vitro study of dynamic cellular and inter-cellular processes, from cell growth and stimulus/response to inter-colony and inter-species signaling.
在微流控装置中使用电沉积响应性海藻酸盐凝胶在生理 pH 条件下实现可编程的 3D 细胞组装，平行侧壁电极可实现对细胞组装的直接观察。证明了电触发组装以及随后哺乳动物细胞的活力，以及不同大肠杆菌菌株的空间可编程、多地址组装。我们的方法能够在体外研究动态细胞和细胞间过程，包括细胞生长和刺激/反应以及菌落间和种间信号传递。
