用于细胞封装和三维细胞培养的单分散、结构均匀的海藻酸盐微凝胶的微流体制备
Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture.
作者信息
Utech Stefanie, Prodanovic Radivoje, Mao Angelo S, Ostafe Raluca, Mooney David J, Weitz David A
机构信息
School of Engineering and Applied Sciences, Harvard University, 02138, Cambridge, MA, USA.
Faculty of Chemistry, University of Belgrade, Studentski trg 12, 11000, Belgrade, Serbia.
出版信息
Adv Healthc Mater. 2015 Aug 5;4(11):1628-33. doi: 10.1002/adhm.201500021. Epub 2015 Jun 3.
Abstract
Monodisperse alginate microgels (10-50 μm) are created via droplet-based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.
摘要
通过一种新型交联程序，利用基于液滴的微流控技术制备出了单分散藻酸盐微凝胶（10 - 50微米）。藻酸盐的离子交联是由螯合钙离子的释放诱导产生的。该过程将液滴形成与凝胶化反应分离，从而能够在温和的反应条件下对尺寸和均匀性进行出色的控制。将活的间充质干细胞封装在生成的三维微环境中并进行培养。
相似文献
1
Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture.用于细胞封装和三维细胞培养的单分散、结构均匀的海藻酸盐微凝胶的微流体制备
Adv Healthc Mater. 2015 Aug 5;4(11):1628-33. doi: 10.1002/adhm.201500021. Epub 2015 Jun 3.
2
Enzymatic Crosslinking of Polymer Conjugates is Superior over Ionic or UV Crosslinking for the On-Chip Production of Cell-Laden Microgels.对于载细胞微凝胶的芯片生产，聚合物共轭物的酶促交联优于离子交联或紫外线交联。
Macromol Biosci. 2016 Oct;16(10):1524-1532. doi: 10.1002/mabi.201600174. Epub 2016 Jul 21.
3
Microfluidic one-step fabrication of radiopaque alginate microgels with in situ synthesized barium sulfate nanoparticles.微流控一步法制备原位合成硫酸钡纳米粒子的不透射线海藻酸钠微凝胶。
Lab Chip. 2012 Nov 21;12(22):4781-6. doi: 10.1039/c2lc40740j.
4
Controllable generation and encapsulation of alginate fibers using droplet-based microfluidics.利用基于液滴的微流控技术可控生成和封装海藻酸盐纤维。
Lab Chip. 2016 Jan 7;16(1):59-64. doi: 10.1039/c5lc01150g.
5
Manipulating the generation of Ca-alginate microspheres using microfluidic channels as a carrier of gold nanoparticles.利用微流控通道作为金纳米颗粒的载体来操控海藻酸钙微球的生成。
Lab Chip. 2006 Jul;6(7):954-7. doi: 10.1039/b606424h. Epub 2006 May 18.
6
Digital electrophoresis of charged droplets.带电液滴的数字电泳。
Anal Chem. 2013 Apr 16;85(8):4038-44. doi: 10.1021/ac303778j. Epub 2013 Mar 29.
7
Shape-controlled production of biodegradable calcium alginate gel microparticles using a novel microfluidic device.使用新型微流控装置可控形状地生产可生物降解的海藻酸钙凝胶微粒。
Langmuir. 2006 Oct 24;22(22):9453-7. doi: 10.1021/la061729+.
8
Using a microfluidic chip and internal gelation reaction for monodisperse calcium alginate microparticles generation.利用微流控芯片和内部凝胶化反应生成单分散海藻酸钙微粒。
Front Biosci. 2007 May 1;12:3061-7. doi: 10.2741/2295.
9
Microfluidic synthesis of tail-shaped alginate microparticles using slow sedimentation.利用缓慢沉降法制备鱼尾状海藻酸钠微球的微流控合成。
Electrophoresis. 2013 Feb;34(3):425-31. doi: 10.1002/elps.201200282. Epub 2013 Jan 6.
10
Microfluidic Production of Alginate Hydrogel Particles for Antibody Encapsulation and Release.用于抗体包封与释放的藻酸盐水凝胶颗粒的微流体制备
Macromol Biosci. 2015 Dec;15(12):1641-6. doi: 10.1002/mabi.201500226. Epub 2015 Jul 21.
引用本文的文献
1
Surfactant-Free Electrosprayed Alginate Beads for Oral Delivery of Hydrophobic Compounds.用于口服递送疏水性化合物的无表面活性剂电喷雾海藻酸盐珠粒
Polymers (Basel). 2025 Jul 30;17(15):2098. doi: 10.3390/polym17152098.
2
Interfacial Engineering-Free Microfluidics: Toward a Mild and Cost-Effective Strategy for Surfactant- and Demulsifier-Free Hydrogel Microsphere Fabrication.无界面工程微流控：迈向一种温和且经济高效的策略，用于制备不含表面活性剂和破乳剂的水凝胶微球
Micromachines (Basel). 2025 Jun 22;16(7):733. doi: 10.3390/mi16070733.
3
Microfluidic generation of bacterial biohybrids for magnetic guidance and content release.用于磁导向和内容物释放的细菌生物杂交体的微流体制备
Chem Commun (Camb). 2025 Jul 14. doi: 10.1039/d5cc00449g.
4
Microfluidic Production of Spatially Structured Biomimetic Microgels as Compartmentalized Artificial Cells.微流控制备具有空间结构的仿生微凝胶作为分隔式人工细胞
Small Sci. 2025 Feb 6;5(4):2400320. doi: 10.1002/smsc.202400320. eCollection 2025 Apr.
5
Indenting at the Microscale: Guidelines for Robust Mechanical Characterization of Alginate Microgels.微观尺度下的缩进：海藻酸钠微凝胶稳健力学表征指南
ACS Appl Mater Interfaces. 2025 Mar 5;17(9):13513-13526. doi: 10.1021/acsami.4c20952. Epub 2025 Feb 24.
6
Tough and self-adhesive zwitterionic hydrogels with mechano-responsive release of bFGF for tympanic membrane repair.具有机械响应性释放碱性成纤维细胞生长因子用于鼓膜修复的坚韧且自粘性两性离子水凝胶。
Mater Today Bio. 2024 Aug 24;28:101212. doi: 10.1016/j.mtbio.2024.101212. eCollection 2024 Oct.
7
Physical and Soluble Cues Enhance Tendon Progenitor Cell Invasion into Injectable Synthetic Hydrogels.物理和可溶性信号增强肌腱祖细胞向可注射合成水凝胶的侵袭。
Adv Funct Mater. 2022 Nov 24;32(48):2207556. doi: 10.1002/adfm.202207556. Epub 2022 Sep 28.
8
Microgels for Cell Delivery in Tissue Engineering and Regenerative Medicine.用于组织工程和再生医学中细胞递送的微凝胶
Nanomicro Lett. 2024 Jun 17;16(1):218. doi: 10.1007/s40820-024-01421-5.
9
Lab on a Particle Technologies.颗粒技术实验室
Anal Chem. 2024 May 21;96(20):7817-7839. doi: 10.1021/acs.analchem.4c01510. Epub 2024 Apr 22.
10
Surface-Functionalized Microgels as Artificial Antigen-Presenting Cells to Regulate Expansion of T Cells.表面功能化微凝胶作为人工抗原呈递细胞调节 T 细胞的扩增。
Adv Mater. 2024 Aug;36(31):e2309860. doi: 10.1002/adma.202309860. Epub 2024 May 29.
本文引用的文献
1
Fabrication of alginate-gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties.海藻酸钠-明胶交联水凝胶微胶囊的制备及其微观结构和理化性质的评估
J Mater Chem B. 2014 Mar 21;2(11):1470-1482. doi: 10.1039/c3tb21509a. Epub 2014 Jan 24.
2
Evaluation of fibroblasts adhesion and proliferation on alginate-gelatin crosslinked hydrogel.成纤维细胞在海藻酸钠-明胶交联水凝胶上的黏附与增殖评估。
PLoS One. 2014 Sep 30;9(9):e107952. doi: 10.1371/journal.pone.0107952. eCollection 2014.
3
Monodisperse alginate microgel formation in a three-dimensional microfluidic droplet generator.在三维微流控液滴发生器中形成单分散的海藻酸钠微凝胶。
Biomicrofluidics. 2012 Nov 7;6(4):44108. doi: 10.1063/1.4765337. eCollection 2012.
4
Shape controllable microgel particles prepared by microfluidic combining external ionic crosslinking.微流控结合外部离子交联制备形状可控的微凝胶颗粒。
Biomicrofluidics. 2012 Jun;6(2):26502-265029. doi: 10.1063/1.4720396. Epub 2012 May 18.
5
Microfluidic encapsulation of cells in polymer microgels.微流控法将细胞包封在聚合物微凝胶中。
Small. 2012 Jun 11;8(11):1633-42. doi: 10.1002/smll.201102464. Epub 2012 Mar 29.
6
A microfluidic approach to encapsulate living cells in uniform alginate hydrogel microparticles.一种微流控方法，用于将活细胞包封在均匀的藻酸盐水凝胶微球中。
Macromol Biosci. 2012 Jul;12(7):946-51. doi: 10.1002/mabi.201100351. Epub 2012 Feb 7.
7
Alginate: properties and biomedical applications.藻酸盐：性质与生物医学应用
Prog Polym Sci. 2012 Jan;37(1):106-126. doi: 10.1016/j.progpolymsci.2011.06.003.
8
Microfluidic fabrication of microengineered hydrogels and their application in tissue engineering.微流控技术制备微工程水凝胶及其在组织工程中的应用。
Lab Chip. 2012 Jan 7;12(1):45-59. doi: 10.1039/c1lc20859d. Epub 2011 Nov 21.
9
The use of micro- and nanospheres as functional components for bone tissue regeneration.微球和纳米球在骨组织再生中的应用：作为功能性成分。
Tissue Eng Part B Rev. 2012 Feb;18(1):24-39. doi: 10.1089/ten.TEB.2011.0184. Epub 2011 Sep 23.
10
Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate.利用牵引力介导的细胞/基质界面操纵来控制干细胞命运。
Nat Mater. 2010 Jun;9(6):518-26. doi: 10.1038/nmat2732. Epub 2010 Apr 25.
Zotero 插件