Foley D H, Wilkerson R C, Cooper R D, Volovsek M E, Bryan J H
Department of Entomology, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
Mol Phylogenet Evol. 2007 Apr;43(1):283-97. doi: 10.1016/j.ympev.2006.10.008. Epub 2006 Oct 17.
The Australasian Annulipes Complex is the most species-rich among Anopheles mosquitoes, with at least 15 sibling species suspected. Members of this complex are the most likely vectors of malaria in the past in southern Australia and are involved in the spread of myxomatosis among rabbits. In this, the first comprehensive molecular study of the Annulipes Complex, 23 ITS2 rDNA variants were detected from collections throughout Australia and Papua New Guinea, including diagnostic variants for the previously identified An. annulipes species A-G. Specimens of each ITS2 variant were sequenced for portions of the mitochondrial COI, COII and nuclear EF-1alpha genes. Partitioned Bayesian and Maximum Parsimony analyses confirmed the monophyly of the Annulipes Complex and revealed at least 17 clades that we designate species A-Q. These species belong to two major clades, one in the north and one mainly in the south, suggesting that climate was a driver of species radiation. We found that 65% (11) of the 17 sibling species recorded here had unique COI sequences, suggesting that DNA barcoding will be useful for diagnosing species within the Annulipes Complex. A comparison of the taxa revealed morphological characters that may be diagnostic for some species. Our results substantially increase the size of the subgenus Cellia in Australasia, and will assist species-level studies of the Annulipes Complex.
澳大拉西亚环足按蚊复合体是按蚊中物种最丰富的，怀疑至少有15个姐妹种。该复合体的成员过去很可能是澳大利亚南部疟疾的传播媒介，并且参与了兔黏液瘤病的传播。在这项对环足按蚊复合体的首次全面分子研究中，从澳大利亚和巴布亚新几内亚各地的样本中检测到23个ITS2核糖体DNA变异体，包括先前鉴定的环足按蚊A - G种的诊断变异体。对每个ITS2变异体的样本进行了线粒体COI、COII和核EF - 1α基因部分的测序。分区贝叶斯分析和最大简约法分析证实了环足按蚊复合体的单系性，并揭示了至少17个分支，我们将其指定为A - Q种。这些物种属于两个主要分支，一个在北部，一个主要在南部，这表明气候是物种辐射的驱动因素。我们发现，这里记录的17个姐妹种中有65%（11个）具有独特的COI序列，这表明DNA条形码将有助于诊断环足按蚊复合体内的物种。对这些分类单元的比较揭示了可能对某些物种具有诊断作用的形态特征。我们的结果大幅增加了澳大拉西亚塞利按蚊亚属的规模，并将有助于对环足按蚊复合体进行物种水平的研究。
