Matrix Science Ltd., London, UK.
J Proteomics. 2011 Sep 6;74(10):1842-51. doi: 10.1016/j.jprot.2011.05.014. Epub 2011 May 15.
The subject of this tutorial is protein identification and characterisation by database searching of MS/MS Data. Peptide Mass Fingerprinting is excluded because it is covered in a separate tutorial. Practical aspects of database searching are emphasised, such as choice of sequence database, effect of mass tolerance, and how to identify post-translational modifications. The relationship between sensitivity and specificity is discussed, as is the challenge of using peptide match information to infer which proteins were present in the sample. Since these tutorials are introductory in nature, most references are to reviews, rather than primary research papers. Some familiarity with mass spectrometry and protein chemistry is assumed. There is an accompanying slide presentation, including speaker notes, and a collection of web-based, practical exercises, designed to reinforce key points. This Tutorial is part of the International Proteomics Tutorial Programme (IPTP 6).
本教程的主题是通过数据库搜索 MS/MS 数据来鉴定和描述蛋白质。由于肽质量指纹图谱已在单独的教程中介绍，因此将不包括该内容。本教程强调数据库搜索的实际方面，例如序列数据库的选择、质量容限的影响以及如何识别翻译后修饰。还讨论了灵敏度和特异性之间的关系，以及如何利用肽匹配信息推断样品中存在哪些蛋白质。由于这些教程本质上是介绍性的，因此大多数参考资料都是综述，而不是原始研究论文。假设读者对质谱和蛋白质化学有一定的了解。本教程有一个配套的幻灯片演示文稿，包括演讲者注释，以及一系列基于网络的实践练习，旨在强化要点。本教程是国际蛋白质组学教程计划 (IPTP 6) 的一部分。
