噬菌体T4基因60编码间隙的核糖体跳跃需要一个新生肽。
A nascent peptide is required for ribosomal bypass of the coding gap in bacteriophage T4 gene 60.
作者信息
Weiss R B, Huang W M, Dunn D M
机构信息
Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah.
出版信息
Cell. 1990 Jul 13;62(1):117-26. doi: 10.1016/0092-8674(90)90245-a.
Abstract
Bacteriophage T4 DNA topoisomerase gene 60 contains a 50 nucleotide untranslated region within the coding sequence of its mRNA. Translational bypass of this sequence by elongating ribosomes has been postulated for the mode of synthesis of an 18 kd polypeptide specified by the split coding segments. Ribosome bypass of the untranslated region also occurs when a segment of gene 60 is fused to lacZ and expressed in E. coli. The efficiency of bypass in these gene 60-lacZ fusions approaches 100%. Here, mutations that delete, insert, or substitute nucleotides from gene 60-lacZ fusions are examined. Essential features necessary for high level gap bypass emerging from this analysis are a cis-acting nascent peptide sequence, a short duplication bordering the gap, and a stop codon contained in a stem-loop structure at the 5' junction of the gap.
摘要
噬菌体T4 DNA拓扑异构酶基因60在其mRNA编码序列内含有一个50个核苷酸的非翻译区。已推测延伸的核糖体对该序列进行翻译跳跃是由分裂编码片段指定的18kd多肽的合成模式。当基因60的一段与lacZ融合并在大肠杆菌中表达时，也会发生核糖体对非翻译区的跳跃。在这些基因60-lacZ融合体中，跳跃效率接近100%。在此，对从基因60-lacZ融合体中缺失、插入或替换核苷酸的突变进行了研究。该分析中出现的高水平缺口跳跃所必需的基本特征是一个顺式作用的新生肽序列、一个与缺口相邻的短重复序列以及一个位于缺口5'连接处茎环结构中的终止密码子。
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