Laboratório de Mutagênese, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG Brazil.
Genet Mol Biol. 2009 Jan;32(1):133-7. doi: 10.1590/S1415-47572009005000008. Epub 2009 Jan 17.
The consumption of organic tomatoes (ORTs) reduces the risk of harmful effects to humans and the environment caused by exposure to toxic agrochemicals. In this study, we used the somatic mutation and recombination test (SMART) of wing spots in Drosophila melanogaster to evaluate the genotoxicity of ORT and the effect of cotreatment with ORT on the genotoxicity of Doxorubicin(®) (DXR, a cancer chemotherapeutic agent) that is mediated by free radical formation. Standard (ST) cross larvae were treated chronically with solutions containing 25%, 50% or 100% of an aqueous extract of ORT, in the absence and presence of DXR (0.125 mg/mL), and the number of mutant spots on the wings of emergent flies was counted. ORT alone was not genotoxic but enhanced the toxicity of DXR when administered concomitantly with DXR. The ORT-enhanced frequency of spots induced by DXR may have resulted from the interaction of ORT with the enzymatic systems that catalyze the metabolic detoxification of this drug.
食用有机番茄（ORTs）可降低人类和环境因接触有毒农用化学品而产生有害影响的风险。在这项研究中，我们使用黑腹果蝇翅膀体突变和重组测试（SMART）来评估 ORT 的遗传毒性，以及 ORT 与多柔比星（DXR，一种癌症化疗药物）共同处理对由自由基形成介导的 DXR 遗传毒性的影响。标准（ST）杂交幼虫用含有 25％、50％或 100％ORT 水提物的溶液进行慢性处理，在不存在和存在 DXR（0.125 mg/mL）的情况下，计算新出现的苍蝇翅膀上突变斑的数量。ORT 本身没有遗传毒性，但与 DXR 同时给药时会增强 DXR 的毒性。ORT 增强 DXR 诱导的斑点频率可能是由于 ORT 与催化该药物代谢解毒的酶系统相互作用所致。
