Yoon Hyeon, Ahn Seunghyun, Kim Geunhyung
Bio/Nanofluidics Lab, Department of Mechanical Engineering, Chosun University, 375 Seosok-dong, Gwangju 501-759, Korea.
Macromol Rapid Commun. 2009 Oct 1;30(19):1632-7. doi: 10.1002/marc.200900264. Epub 2009 Jul 10.
A hybrid technology that combines a three-dimensional (3-D) dispensing system with an electrospinning process was used to produce a hierarchical 3-D scaffold consisting of micro-sized polycaprolactone (PCL) strands and micro/nano-sized fibres. The micro/nanofibre biocomposites electrospun with PCL/small intestine submucosa (SIS) and PCL/Silk fibroin were layered between melt-plotted micro-strands. The scaffold containing SIS exhibited a stronger hydrophilic property than other scaffolds due to the various hydrophilic components in SIS. The 3-D hierarchical scaffold having biocomposites exhibited an incredibly enhanced initial cell attachment and proliferation of bone marrow-derived mesenchymal stem cells relative to the normally designed 3-D scaffold.
一种将三维（3-D） dispensing系统与静电纺丝工艺相结合的混合技术被用于制造一种分层的三维支架，该支架由微米级聚己内酯（PCL）股线和微米/纳米级纤维组成。用PCL/小肠黏膜下层（SIS）和PCL/丝素蛋白静电纺丝的微/纳米纤维生物复合材料被分层置于热熔微股线之间。由于SIS中存在各种亲水性成分，含有SIS的支架表现出比其他支架更强的亲水性。与常规设计的三维支架相比，具有生物复合材料的三维分层支架在骨髓间充质干细胞的初始细胞附着和增殖方面表现出极大的增强。
