TNO Microbiology and Systems Biology, 3700 AJ, Zeist, The Netherlands.
Annu Rev Microbiol. 2011;65:57-69. doi: 10.1146/annurev.micro.112408.134009.
For more than a century, filamentous fungi have been used for the production of a wide variety of endogenous enzymes of industrial interest. More recently, with the use of genetic engineering tools developed for these organisms, this use has expanded for the production of nonnative heterologous proteins. In this review, an overview is given of examples describing the production of a special class of these proteins, namely chimeric proteins. The production of two types of chimeric proteins have been explored: (a) proteins grafted for a specific substrate-binding domain and (b) fusion proteins containing two separate enzymatic activities. Various application areas for the use of these chimeric proteins are described.
一个多世纪以来，丝状真菌一直被用于生产各种具有工业应用价值的内源性酶。最近，随着为这些生物开发的遗传工程工具的使用，这种用途已经扩展到生产非天然的异源蛋白。在这篇综述中，概述了描述生产一类特殊蛋白，即嵌合蛋白的例子。已经探索了两种类型的嵌合蛋白的生产：(a) 接枝有特定底物结合域的蛋白和(b) 包含两种独立酶活性的融合蛋白。描述了这些嵌合蛋白的各种应用领域。
