基因多态性通过 MLST 鉴定了 1953 年至 2013 年间在中国分离的布鲁氏菌种/生物型。
Genetic polymorphisms identify in species/biovars of Brucella isolated in China between 1953 and 2013 by MLST.
机构信息
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, MOA, Qingdao, China.
出版信息
BMC Microbiol. 2018 Jan 10;18(1):7. doi: 10.1186/s12866-018-1149-0.
BACKGROUND
Brucellosis incidence in China is divided into three stages: high incidence (1950s-1960s), decline (1970s-1980s), and re-emergence (1990s-2010s). At the re-emergence stage, Brucellosis incidence grew exponentially and spread to all 32 provinces. We describe the magnitude and the etiological distribution changes in mainland China by genotyping data and emphasize its recent reemergence. We also provide the genetic diversity and molecular epidemiological characteristics of Brucella.
RESULTS
From a total of 206 Brucella isolates, 19 MLST genotypes (STs) were identified and 13 new STs(ST71-83)were found. MLST grouped the population into three clusters. B. melitensis, B. abortus and B. suis were grouped into cluster 1, 2 and 3 respectively. The predominant genotype in the first cluster by MLST, remained unchanged during the three stages. However, the proportion of genotypes in the three stages had changed. More isolates were clustered in ST8 at the re-emergence stage. STs71-74, which were not found in the two former stages, appeared at the re-emergence stage.
CONCLUSIONS
The changing molecular epidemiology of brucellosis improve our understanding of apparent geographic expansion from the historically affected north of China to southern provinces in recent reemergence.
背景
中国布鲁氏菌病的发病率分为三个阶段：高发期（20 世纪 50 年代至 60 年代）、下降期（70 年代至 80 年代）和再现期（90 年代至 21 世纪 10 年代）。在再现期，布鲁氏菌病发病率呈指数级增长，并蔓延至全国 32 个省。我们通过基因分型数据描述了中国内地的发病规模和病因分布变化，并强调了其最近的再现。我们还提供了布鲁氏菌的遗传多样性和分子流行病学特征。
结果
从总共 206 株布鲁氏菌分离株中，鉴定出 19 种 MLST 基因型（ST），并发现了 13 种新的 ST（ST71-83）。MLST 将人群分为三个聚类。B. melitensis、B. abortus 和 B. suis 分别归入聚类 1、2 和 3。通过 MLST，第一聚类中的主要基因型在三个阶段保持不变。然而，三个阶段的基因型比例发生了变化。在再现期，更多的分离株聚类在 ST8 中。在前两个阶段未发现的 STs71-74 出现在再现期。
结论
布鲁氏菌病不断变化的分子流行病学提高了我们对明显地理扩张的认识，从历史上受影响的中国北方到最近再现期的南方省份。
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