Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117312 Moscow, Russia.
Bioengineering Center, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/1, 117312 Moscow, Russia.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):890-894. doi: 10.1099/ijs.0.027623-0. Epub 2011 Jun 3.
A thermophilic, anaerobic, dissimilatory Mn(IV)- and Fe(III)-reducing bacterium (strain SLM 61T) was isolated from a terrestrial hot spring on the Kamchatka peninsula. The cells were straight rods, 0.5-0.6 µm in diameter and 1.0-6.0 µm long, and exhibited tumbling motility by means of peritrichous flagellation. The strain grew at 26-70 °C, with an optimum at 58-60 °C, and at pH 5.5-8.0, with an optimum at pH 6.5. Growth of SLM 61T was observed at 0-2.0 % (w/v) NaCl, with an optimum at 0.5 % (w/v). The generation time under optimal growth conditions was 40 min. Strain SLM 61T grew and reduced Mn(IV), Fe(III) or nitrate with a number of organic acids and complex proteinaceous compounds as electron donors. It was capable of chemolithoautotrophic growth using molecular hydrogen as an electron donor, Fe(III) but not Mn(IV) or nitrate as an electron acceptor and CO2 as a carbon source. It also was able to ferment pyruvate, yeast extract, glucose, fructose, sucrose and maltose. The G+C content of DNA of strain SLM 61T was 50.9 mol%. 16S rRNA gene sequence analysis revealed that the closest relative of the isolated organism was Carboxydocella thermautotrophica 41T (96.9 % similarity). On the basis of its physiological properties and phylogenetic analyses, the isolate is considered to represent a novel species, for which the name Carboxydocella manganica sp. nov. is proposed. The type strain is SLM 61T (=DSM 23132T=VKM B-2609T). C. manganica is the first described representative of the genus Carboxydocella that possesses the ability to reduce metals and does not utilize CO.
一株嗜热、厌氧、异化锰(IV)和铁(III)还原细菌（菌株 SLM 61T）是从堪察加半岛的一个陆地温泉中分离得到的。细胞呈直杆状，直径 0.5-0.6μm，长 1.0-6.0μm，通过周生鞭毛的翻滚运动表现出滚动运动。该菌株在 26-70°C 下生长，最佳温度为 58-60°C，在 pH5.5-8.0 下生长，最佳 pH 为 6.5。在 0-2.0%（w/v）NaCl 下观察到 SLM 61T 的生长，最佳浓度为 0.5%（w/v）。在最佳生长条件下，代时为 40 分钟。SLM 61T 可以利用多种有机酸和复杂的蛋白质化合物作为电子供体生长并还原 Mn(IV)、Fe(III)或硝酸盐。它能够以分子氢作为电子供体、Fe(III)而不是 Mn(IV)或硝酸盐作为电子受体和 CO2 作为碳源进行化能自养生长。它还能够发酵丙酮酸、酵母提取物、葡萄糖、果糖、蔗糖和麦芽糖。菌株 SLM 61T 的 DNA G+C 含量为 50.9mol%。16S rRNA 基因序列分析表明，分离出的生物与 Carboxydocella thermautotrophica 41T 最为接近（相似度为 96.9%）。基于其生理特性和系统发育分析，该分离株被认为代表一个新物种，建议将其命名为 Carboxydocella manganica sp. nov。模式株为 SLM 61T（=DSM 23132T=VKM B-2609T）。C. manganica 是第一个被描述的具有还原金属能力且不利用 CO 的 Carboxydocella 属代表。
