Gerna G, Sarasini A, Zentilin L, Di Matteo A, Miranda P, Parea M, Battaglia M, Milanesi G
Virus Laboratory, University of Pavia, Irees Policlinico S. Matteo, Italy.
Arch Virol. 1990;112(1-2):27-40. doi: 10.1007/BF01348983.
During an epidemiological study on the prevalence of human rotavirus (HRV) serotypes 1-4 in Europe, we found that some strains could not be typed. However, when a monoclonal antibody directed to serotype 8 HRV was included in the typing assay, we detected seven 69 M-like (serotype 8) strains, six from Finland and one from Italy. The previously reported serotype 8 HRV strains, 69 M, B 37, and B 38 isolated in Indonesia, were of subgroup I specificity and presented a peculiar "super short" RNA electropherotype. In contrast, all the seven European strains possessed a long RNA pattern, and one of them had subgroup II specificity. Three of these strains were adapted to growth in cell cultures and were further characterized by neutralization and by Northern blot hybridization. They appeared to be closely related to serotype 8 HRV strain 69 M by neutralization, but showed partial homology with several human and animal strains by hybridization. The epidemiological importance of these serotype 8 strains circulating in Europe should be investigated, in view of their possible inclusion in a rotavirus vaccine.
在一项关于欧洲人轮状病毒（HRV）1 - 4血清型流行情况的流行病学研究中，我们发现一些毒株无法分型。然而，当在分型检测中加入针对8型HRV的单克隆抗体时，我们检测到了7株69M样（8型）毒株，其中6株来自芬兰，1株来自意大利。之前在印度尼西亚分离出的8型HRV毒株69M、B37和B38具有I亚组特异性，并呈现出一种特殊的“超短”RNA电泳图谱。相比之下，所有7株欧洲毒株都具有长RNA图谱，其中1株具有II亚组特异性。其中3株毒株适应在细胞培养物中生长，并通过中和试验和Northern印迹杂交进一步进行了鉴定。通过中和试验，它们似乎与8型HRV毒株69M密切相关，但通过杂交显示与几种人和动物毒株存在部分同源性。鉴于这些8型毒株可能会被纳入轮状病毒疫苗，应调查它们在欧洲传播的流行病学重要性。
