Kobayashi N, Lintag I C, Urasawa T, Taniguchi K, Saniel M C, Urasawa S
Department of Hygiene and Epidemiology, Sapporo Medical College, Japan.
Arch Virol. 1989;109(1-2):11-23. doi: 10.1007/BF01310514.
During an epidemiological study of human rotavirus in Metro Manila, Philippines, we found 20 unusual strains which belong to subgroup I but have "long" RNA pattern typical of subgroup II human rotavirus. The RNA patterns of the 20 strains were classified into four groups though they were very similar to each other. Four strains, designated L4, L26, L27, and L34 were isolated in MA104 cells from stool specimens. They possessed subgroup I specificity and long RNA pattern identical to that of the viruses in the original stool samples. The serotype specificity of these strains could not be determined by either enzyme-linked immunosorbent assay (ELISA) or neutralization test, while one strain (L27) was neutralized by serotype 2-specific anti-VP4 monoclonal antibody. These strains were suggested to have an unusual antigenicity on VP7.
在菲律宾马尼拉大都会进行的一项人类轮状病毒流行病学研究中，我们发现了20株不寻常的毒株，它们属于I亚组，但具有II亚组人类轮状病毒典型的“长”RNA模式。尽管这20株毒株的RNA模式彼此非常相似，但仍被分为四组。从粪便标本中在MA104细胞中分离出四株毒株，分别命名为L4、L26、L27和L34。它们具有I亚组特异性，且其长RNA模式与原始粪便样本中的病毒相同。这些毒株的血清型特异性无法通过酶联免疫吸附测定（ELISA）或中和试验确定，而其中一株毒株（L27）被2型特异性抗VP4单克隆抗体中和。这些毒株被认为在VP7上具有不寻常的抗原性。
