Iwasaki H, Shiba T, Nakata A, Shinagawa H
Department of Experimental Chemotherapy, Osaka University, Japan.
Mol Gen Genet. 1989 Oct;219(1-2):328-31. doi: 10.1007/BF00261196.
The ruv operon of Escherichia coli consists of two genes, orf1 and ruv, which encode 22 and 37 kilodalton proteins, respectively, and are regulated by the SOS system. Although the distal gene, ruv, is known to be involved in DNA repair, the function of orf1 has not been studied. To examine whether orf1 is also involved in DNA repair, we constructed a strain with a deletion of the entire ruv operon. The strain was sensitive to UV even after introduction of low copy number plasmids carrying either orf1 or ruv, but UV resistance was restored by introduction of a plasmid carrying both orf1 and ruv. These results suggest that orf1 as well as ruv is involved in DNA repair. Therefore, orf1 and ruv should be renamed ruvA and ruvB, respectively.
大肠杆菌的ruv操纵子由两个基因组成，即orf1和ruv，它们分别编码22千道尔顿和37千道尔顿的蛋白质，并受SOS系统调控。尽管已知远端基因ruv参与DNA修复，但orf1的功能尚未得到研究。为了检测orf1是否也参与DNA修复，我们构建了一个缺失整个ruv操纵子的菌株。即使导入携带orf1或ruv的低拷贝数质粒后，该菌株对紫外线仍敏感，但导入同时携带orf1和ruv的质粒后，其紫外线抗性得以恢复。这些结果表明，orf1和ruv都参与DNA修复。因此，orf1和ruv应分别重新命名为ruvA和ruvB。
