Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695, USA.
Appl Biochem Biotechnol. 2011 Oct;165(3-4):832-44. doi: 10.1007/s12010-011-9301-4. Epub 2011 Jun 7.
In this study, a strategy to reduce enzyme dosage is evaluated by applying two post-treatments, oxygen delignification and mechanical refining. The sugar conversion for GL12 substrates was increased from 51.5% to 77.9% with post-treatments at the enzyme dosage of 10 FPU. When the amount of enzyme was reduced to 5 FPU with post-treatments, the conversion of 71.8% was obtained, which was significant higher than the conversion without any post-treatment using 10 FPU (51.5%). This clearly demonstrates the benefit of post-treatments that allows more than 50% of enzyme reduction at the same level of enzymatic conversion. Enzyme-accessible surface area and pore volume were evaluated by Simons' staining and DSC thermoporometry methods, and strong correlations were found with the sugar conversion.
在这项研究中，通过应用两种后处理方法——氧脱木素和机械精磨，评估了一种减少酶用量的策略。在酶用量为 10 FPU 的情况下，GL12 底物的糖转化率从 51.5%增加到 77.9%。当用后处理将酶用量减少到 5 FPU 时，转化率为 71.8%，显著高于不使用任何后处理用 10 FPU（51.5%）的转化率。这清楚地表明了后处理的好处，它允许在相同的酶转化水平上减少 50%以上的酶用量。通过 Simons 染色和 DSC 热压法评估了酶可及表面积和孔体积，发现与糖转化率有很强的相关性。
