Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, USA.
Dev Dyn. 2011 Jul;240(7):1826-40. doi: 10.1002/dvdy.22669. Epub 2011 Jun 3.
Urodele amphibians are unique among adult vertebrates in their ability to regenerate missing limbs. The process of limb regeneration requires several key tissues including a regeneration-competent wound epidermis called the regeneration epithelium (RE). We used microarray analysis to profile gene expression of the RE in the axolotl, a Mexican salamander. A list of 125 genes and expressed sequence tags (ESTs) showed a ≥1.5-fold expression in the RE than in a wound epidermis covering a lateral cuff wound. A subset of the RE ESTs and genes were further characterized for expression level changes over the time-course of regeneration. This study provides the first large scale identification of specific gene expression in the RE.
有尾两栖动物在成体脊椎动物中具有独特的能力，可以再生缺失的肢体。肢体再生的过程需要几个关键组织，包括一个再生能力的伤口表皮，称为再生上皮（RE）。我们使用微阵列分析对蝾螈，一种墨西哥蝾螈的 RE 进行了基因表达谱分析。有 125 个基因和表达序列标签（EST）的列表显示，RE 的表达水平比覆盖侧袖口伤口的伤口表皮高 1.5 倍以上。RE 的 EST 和基因的一部分进一步被表征为在再生过程中的时间过程中表达水平的变化。这项研究首次大规模鉴定了 RE 中的特定基因表达。
