Cell Dynamics Research Center, Research Center for Biomolecular Nanotechnology, School of Life Sciences, Gwangju Institute of Science and Technology, 261 Chemdangwagi-ro, Gwangju 500-712, Republic of Korea.
Small. 2011 Aug 8;7(15):2241-9. doi: 10.1002/smll.201100472. Epub 2011 Jun 7.
CG-rich duplex containing prostate-specific membrane antigen (PSMA) aptamer-conjugated thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPIONs) is reported as prostate cancer-specific nanotheranostic agents. These agents are capable of prostate tumor detection in vivo by magnetic resonance imaging (MRI) and selective delivery of drugs to the tumor tissue, simultaneously. The prepared PSMA-functionalized TCL-SPION via a hybridization method (Apt-hybr-TCL-SPION) exhibited preferential binding towards target prostate-cancer cells (LNCaP, PSMA+) in both in vitro and in vivo when analyzed by T(2) -weighted MRI. After Dox molecules were loaded onto the Apt-hybr-TCL-SPION through the intercalation of Dox to the CG-rich duplex containing PSMA aptamer as well as electrostatic interaction between the Dox-and-polymer coating layer of the nanoparticles, the resulting Dox@Apt-hybr-TCL-SPION showed selective drug-delivery efficacy in the LNCaP xenograft mouse model. These results suggest that Dox@Apt-hybr-TCL-SPION has potential for use as novel prostate cancer-specific nanotheranostics.
含有富含 CG 的双链体的前列腺特异性膜抗原 (PSMA) 适体偶联的热交联超顺磁性氧化铁纳米粒子 (TCL-SPION) 被报道为前列腺癌特异性纳米治疗剂。这些试剂能够通过磁共振成像 (MRI) 在体内检测前列腺肿瘤，并同时将药物选择性地递送到肿瘤组织。通过杂交方法制备的 PSMA 功能化 TCL-SPION（Apt-hybr-TCL-SPION）在体外和体内通过 T(2) 加权 MRI 分析时，均表现出对靶前列腺癌细胞 (LNCaP、PSMA+) 的优先结合。通过将 Dox 分子插入含有 PSMA 适体的富含 CG 的双链体以及纳米粒子的 Dox 和聚合物涂层之间的静电相互作用，将 Dox 加载到 Apt-hybr-TCL-SPION 上之后，所得的 Dox@Apt-hybr-TCL-SPION 在 LNCaP 异种移植小鼠模型中显示出选择性的药物递送功效。这些结果表明，Dox@Apt-hybr-TCL-SPION 具有作为新型前列腺癌特异性纳米治疗剂的潜力。
