在三个临床实验室中，用于检测巨细胞病毒的定量实时 PCR 分析测量范围内的精密度。
Precision across the analytical measuring range of a quantitative real-time PCR assay for cytomegalovirus detection among three clinical laboratories.
机构信息
Molecular Microbiology Laboratory, Mayo Medical Laboratories New England, 160 Dascomb Rd., Andover, MA 01810, USA.
出版信息
J Clin Microbiol. 2011 Aug;49(8):3044-6. doi: 10.1128/JCM.00486-11. Epub 2011 Jun 8.
Abstract
This study measured the precision of a quantitative laboratory-developed real-time PCR test for cytomegalovirus performed at three different clinical laboratories that use the same methodology. The overall standard deviation (adjusted for analyte level) was 0.18 log(10) copies/ml, and there was no significant relationship between standard deviation and analytical measuring range.
摘要
本研究测量了三个使用相同方法学的不同临床实验室进行的定量实验室开发的实时 PCR 检测巨细胞病毒的精密度。总体标准差（根据分析物水平调整）为 0.18 log(10) 拷贝/ml，且标准差与分析测量范围之间无显著关系。
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