从腹泻的地中海型水牛犊（Bubalus bubalis）中分离出的肠产毒性大肠杆菌（ETEC）、志贺毒素产生大肠杆菌（STEC）和坏死性大肠杆菌（NTEC）的特性。
Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalus bubalis).
机构信息
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055 Portici, NA, Italy.
出版信息
Res Vet Sci. 2012 Aug;93(1):18-22. doi: 10.1016/j.rvsc.2011.05.009. Epub 2011 Jun 11.
Abstract
Two hundred and twenty Escherichia coli isolates from 314 Mediterranean water buffalo calves less than 4 weeks old affected by severe diarrhoea with a lethal outcome were characterized for the presence of the virulence factors LT, ST, Stx1, Stx2, haemolysins, intimin, CNF1, CNF2, CDT-I, CDT-II, CDT-III, CDT-IV, and F17-related fimbriae (F17a, F17b, F17c, F17d). The prevalence of ETEC, STEC and NTEC were 1.8%, 6.8% and 20.9%, respectively. The ETEC isolates were all LT-positive and ST-negative. The STEC isolates were all Stx and intimin-positive, with Stx1 (80%) more frequent than Stx2 (27%). The NTEC isolates were all CNF and Hly-positive, with CNF2 (83%) more frequent than CNF1 (22%). Susceptibility assays to 11 antimicrobials displayed high rates of resistance (>30%) to antimicrobials tested. These data show that the most prevalent strains in diarrhoeic water buffalo calves were NTEC, mostly CNF2 and HlyA-positive, with strong associations CNF2/CDT-III and CNF2/F17c.
摘要
从 314 头小于 4 周龄、患有致死性严重腹泻的地中海型水牛肉牛中分离出 220 株大肠杆菌，对这些分离株的 LT、ST、Stx1、Stx2、溶血素、侵袭素、CNF1、CNF2、CDT-I、CDT-II、CDT-III、CDT-IV 和 F17 相关菌毛（F17a、F17b、F17c、F17d）等毒力因子进行了特征分析。肠产毒性大肠杆菌（ETEC）、肠出血性大肠杆菌（STEC）和肠致病性大肠杆菌（NTEC）的流行率分别为 1.8%、6.8%和 20.9%。ETEC 分离株均为 LT 阳性和 ST 阴性。STEC 分离株均为 Stx 和侵袭素阳性，Stx1（80%）比 Stx2（27%）更为常见。NTEC 分离株均为 CNF 和 Hly 阳性，CNF2（83%）比 CNF1（22%）更为常见。对 11 种抗菌药物的药敏试验显示，受试抗菌药物的耐药率（>30%）均较高。这些数据表明，腹泻水牛肉牛中最常见的菌株是 NTEC，主要为 CNF2 和 HlyA 阳性，与 CNF2/CDT-III 和 CNF2/F17c 有很强的关联。
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本文引用的文献
1
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Nat Rev Microbiol. 2010 Jan;8(1):26-38. doi: 10.1038/nrmicro2265.
2
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J Appl Microbiol. 2009 Feb;106(2):455-8. doi: 10.1111/j.1365-2672.2008.04013.x.
3
Pathophysiology of diarrhea in calves.犊牛腹泻的病理生理学
Vet Clin North Am Food Anim Pract. 2009 Mar;25(1):13-36, xi. doi: 10.1016/j.cvfa.2008.10.013.
4
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Vet Microbiol. 2009 Mar 30;135(3-4):401-5. doi: 10.1016/j.vetmic.2008.09.076. Epub 2008 Oct 4.
5
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Appl Environ Microbiol. 2008 Sep;74(17):5414-21. doi: 10.1128/AEM.00854-08. Epub 2008 Jul 18.
6
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FEMS Microbiol Lett. 2008 Jan;278(2):193-9. doi: 10.1111/j.1574-6968.2007.00991.x. Epub 2007 Dec 5.
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