Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible, 14080 Córdoba, Spain.
Biochem Genet. 2011 Dec;49(11-12):735-47. doi: 10.1007/s10528-011-9446-4. Epub 2011 Jun 10.
In this work, gliadin composition has been analyzed in 33 accessions of durum wheat using MALDI-TOF MS and compared with A-PAGE results. The MALDI-TOF MS spectra were 29,900-42,500 Da, which corresponds to the α/β- and γ-gliadin regions in A-PAGE. The average of gliadin peaks per line was 23 for MALDI-TOF MS and only 14.8 bands for A-PAGE. MALDI-TOF MS identified 33 gliadin peaks in the durum wheat collection, 20 of which were unique peaks present in 7 lines. A-PAGE analysis identified 30 bands, of which only 4 were unique. Thus, the MALDI-TOF MS method was more sensitive than A-PAGE for identifying α/β- and γ-gliadins in the 33 durum wheat lines studied. Phylogenetic analyses performed using MALDI-TOF MS data assigned the durum wheat lines to two groups. The utility of MALDI-TOF MS to determine relationships among genotypes and for identification of durum wheat accessions is discussed.
本工作采用 MALDI-TOF MS 分析了 33 份硬粒小麦的醇溶蛋白组成，并与 A-PAGE 结果进行了比较。MALDI-TOF MS 谱在 29900-42500 Da，对应于 A-PAGE 的α/β-和γ-醇溶蛋白区。MALDI-TOF MS 每条线的醇溶蛋白峰的平均值为 23，而 A-PAGE 仅为 14.8 条带。MALDI-TOF MS 在硬粒小麦中鉴定出 33 个醇溶蛋白峰，其中 20 个是 7 条线中特有的峰。A-PAGE 分析鉴定出 30 条带，其中只有 4 条是特有的。因此，MALDI-TOF MS 法比 A-PAGE 更灵敏地鉴定了所研究的 33 份硬粒小麦醇溶蛋白的α/β-和γ-醇溶蛋白。利用 MALDI-TOF MS 数据进行的系统进化分析将硬粒小麦系分为两组。讨论了 MALDI-TOF MS 用于确定基因型之间的关系和鉴定硬粒小麦品系的用途。
