National Veterinary Institute, Technical University of Denmark, Hangevej 2, DK-8200, Aarhus N, Denmark.
J Med Entomol. 2011 May;48(3):608-14. doi: 10.1603/me11017.
Avian influenza caused by avian influenza virus (AIV) has a negative impact on poultry production. Low-pathogenic AIV (LPAIV) is naturally present in wild birds, and the introduction of the virus into domestic poultry is assumed to occur through contact with wild birds and by human activity, including the movement of live and dead poultry, and fomites such as clothing and vehicles. At present, the possible role of insects in the spread of AIV is dubious. The objective of the present work was to investigate the potential transmission of LPAIV by persistence of the virus in the alimentary tract of house flies, Musca domestica L. (Diptera: Muscidae). Flies were fed three virus concentrations of two AIV strains and then incubated at different temperatures for up to 24 h. The persistence of the two virus strains in the flies declined with increasing incubation temperatures and incubation periods. Similarly, increased virus uptake by the flies increased the persistence of virus. Persistence of infective AIV in flies differed significantly between the two virus strains. The laboratory experiments of the present study indicate that the house fly can be a potential carrier of AIV.
禽流感由禽流感病毒（AIV）引起，对家禽生产造成负面影响。低致病性禽流感（LPAIV）自然存在于野生鸟类中，病毒被认为是通过与野生鸟类接触以及人类活动（包括活禽和死禽的运输以及衣物和车辆等媒介物）传入家禽的。目前，昆虫在 AIV 传播中的可能作用尚不确定。本研究的目的是调查 LPAIV 通过病毒在家蝇（Musca domestica L.）消化道中的持续存在而传播的可能性。苍蝇被喂食两种 AIV 株的三个病毒浓度，然后在不同温度下孵育长达 24 小时。随着孵育温度和孵育时间的增加，两种病毒株在家蝇中的持续存在减少。同样，苍蝇对病毒的摄取增加也会增加病毒的持续存在。两种病毒株在苍蝇中感染性 AIV 的持续时间存在显著差异。本研究的实验室实验表明，家蝇可能是 AIV 的潜在载体。
