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Synthesis of the herpes simplex virus type 1 trans-activator Vmw65 in insect cells using a baculovirus vector.

作者信息

Capone J P, Werstuck G

机构信息

Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.

出版信息

Mol Cell Biochem. 1990 Apr 18;94(1):45-52. doi: 10.1007/BF00223561.

DOI:10.1007/BF00223561
PMID:2166232
Abstract

Vmw65, the Herpes Simplex Virus trans-activator of immediate-early genes, was expressed in insect cells using a recombinant baculovirus expression vector and partially purified. Insect cell-derived Vmw65 was shown to be indistinguishable from authentic Vmw65 present in purified HSV-1 virions based on electrophoretic mobility, immunoreactivity with a monoclonal antibody, and ability to interact with cellular factors to form a protein/DNA complex with oligonucleotides containing a TAATGARAT element.

摘要

相似文献

1
Synthesis of the herpes simplex virus type 1 trans-activator Vmw65 in insect cells using a baculovirus vector.
Mol Cell Biochem. 1990 Apr 18;94(1):45-52. doi: 10.1007/BF00223561.
2
Enhanced infectivity of herpes simplex virus type 1 viral DNA in a cell line expressing the trans-inducing factor Vmw65.在表达反式诱导因子Vmw65的细胞系中，单纯疱疹病毒1型病毒DNA的感染性增强。
J Virol. 1990 Mar;64(3):984-91. doi: 10.1128/JVI.64.3.984-991.1990.
3
The different competitive abilities of viral TAATGARAT elements and cellular octamer motifs, mediate the induction of viral immediate-early genes and the repression of the histone H2B gene in herpes simplex virus infected cells.病毒TAATGARAT元件与细胞八聚体基序不同的竞争能力，介导单纯疱疹病毒感染细胞中病毒立即早期基因的诱导及组蛋白H2B基因的抑制。
Nucleic Acids Res. 1989 Nov 11;17(21):8533-42. doi: 10.1093/nar/17.21.8533.
4
Identification of a domain of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation through the use of protein A fusion proteins.通过使用蛋白A融合蛋白鉴定单纯疱疹病毒反式激活因子Vmw65中形成蛋白质-DNA复合物所需的结构域。
J Virol. 1989 Dec;63(12):5509-13. doi: 10.1128/JVI.63.12.5509-5513.1989.
5
The herpes simplex virus protein Vmw65 can trans-activate both viral and cellular promoters in neuronal cells.单纯疱疹病毒蛋白Vmw65可在神经元细胞中反式激活病毒和细胞启动子。
Biochem J. 1990 Oct 1;271(1):273-6. doi: 10.1042/bj2710273.
6
Herpes simplex virus DNA replication: the UL9 gene encodes an origin-binding protein.
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5414-8. doi: 10.1073/pnas.85.15.5414.
7
Sequence, function, and regulation of the Vmw65 gene of herpes simplex virus type 2.单纯疱疹病毒2型Vmw65基因的序列、功能及调控
J Virol. 1991 Dec;65(12):6705-13. doi: 10.1128/JVI.65.12.6705-6713.1991.
8
Structural requirements in the herpes simplex virus type 1 transactivator Vmw65 for interaction with the cellular octamer-binding protein and target TAATGARAT sequences.单纯疱疹病毒1型反式激活因子Vmw65与细胞八聚体结合蛋白及靶TAATGARAT序列相互作用的结构要求。
J Virol. 1990 Jun;64(6):2716-24. doi: 10.1128/JVI.64.6.2716-2724.1990.
9
Mutational analysis of the herpes simplex virus type 1 trans-inducing factor Vmw65.单纯疱疹病毒1型反式诱导因子Vmw65的突变分析
J Gen Virol. 1988 Oct;69 ( Pt 10):2595-605. doi: 10.1099/0022-1317-69-10-2595.
10
Immediate early and functional AP-1 cis-response elements are involved in the transcriptional regulation of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10).即刻早期和功能性AP-1顺式反应元件参与单纯疱疹病毒2型核糖核苷酸还原酶大亚基（ICP10）的转录调控。
Virus Res. 1992 May;23(3):253-70. doi: 10.1016/0168-1702(92)90112-m.

本文引用的文献

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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
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Characterization of the herpes simplex virion-associated factor responsible for the induction of alpha genes.负责诱导α基因的单纯疱疹病毒粒子相关因子的特性分析
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Differentiation between alpha promoter and regulator regions of herpes simplex virus 1: the functional domains and sequence of a movable alpha regulator.
单纯疱疹病毒1型α启动子与调控区域的区分：一个可移动α调控因子的功能结构域与序列
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Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters.单纯疱疹病毒α基因的调控：由胸苷激酶与α基因启动子融合产生的嵌合基因的表达。
Cell. 1981 May;24(2):555-65. doi: 10.1016/0092-8674(81)90346-9.
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Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription.鉴定编码负责刺激即刻早期转录的反式作用多肽的单纯疱疹病毒DNA序列。
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A small-scale procedure for preparation of nuclear extracts that support efficient transcription and pre-mRNA splicing.一种用于制备支持高效转录和前体mRNA剪接的核提取物的小规模方法。
Gene Anal Tech. 1988 Mar-Apr;5(2):22-31. doi: 10.1016/0735-0651(88)90023-4.
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Signals important for high-level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors.对苜蓿银纹夜蛾核型多角体病毒表达载体中外源基因高水平表达重要的信号。
Virology. 1988 Nov;167(1):56-71. doi: 10.1016/0042-6822(88)90054-2.
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A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.一种与免疫球蛋白基因转录控制元件中的保守序列基序结合的核因子。
Nature. 1986;319(6049):154-8. doi: 10.1038/319154a0.
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The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1.单纯疱疹病毒1型的65,000道尔顿DNA结合及病毒体反式诱导蛋白
J Virol. 1987 Aug;61(8):2428-37. doi: 10.1128/JVI.61.8.2428-2437.1987.
10
DNA sequence of the herpes simplex virus type 1 gene whose product is responsible for transcriptional activation of immediate early promoters.单纯疱疹病毒1型基因的DNA序列，其产物负责立即早期启动子的转录激活。
Nucleic Acids Res. 1985 Nov 11;13(21):7865-79. doi: 10.1093/nar/13.21.7865.