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通过使用蛋白A融合蛋白鉴定单纯疱疹病毒反式激活因子Vmw65中形成蛋白质-DNA复合物所需的结构域。

Identification of a domain of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation through the use of protein A fusion proteins.

作者信息

Werstuck G, Capone J P

机构信息

Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.

出版信息

J Virol. 1989 Dec;63(12):5509-13. doi: 10.1128/JVI.63.12.5509-5513.1989.

DOI:10.1128/JVI.63.12.5509-5513.1989
PMID:2555571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC251227/
Abstract

In order to identify structural domains of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation, subfragments of Vmw65 were expressed in Escherichia coli as fusion polypeptides with protein A of Staphylococcus aureus, and the purified hybrids were used in a band shift assay. The results indicate that a region near the amino terminus of Vmw65 between amino acids 141 and 185 is necessary for complex formation.

摘要

为了鉴定单纯疱疹病毒反式激活因子Vmw65形成蛋白质-DNA复合物所需的结构域，Vmw65的亚片段在大肠杆菌中作为与金黄色葡萄球菌蛋白A的融合多肽表达，并且纯化的杂交体用于凝胶迁移试验。结果表明，Vmw65氨基酸141至185之间靠近氨基末端的区域是复合物形成所必需的。

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Identification of a domain of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation through the use of protein A fusion proteins.通过使用蛋白A融合蛋白鉴定单纯疱疹病毒反式激活因子Vmw65中形成蛋白质-DNA复合物所需的结构域。
J Virol. 1989 Dec;63(12):5509-13. doi: 10.1128/JVI.63.12.5509-5513.1989.
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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
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Characterization of the herpes simplex virion-associated factor responsible for the induction of alpha genes.负责诱导α基因的单纯疱疹病毒粒子相关因子的特性分析
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Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription.鉴定编码负责刺激即刻早期转录的反式作用多肽的单纯疱疹病毒DNA序列。
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The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1.单纯疱疹病毒1型的65,000道尔顿DNA结合及病毒体反式诱导蛋白
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Host cell proteins bind to the cis-acting site required for virion-mediated induction of herpes simplex virus 1 alpha genes.宿主细胞蛋白与病毒体介导的单纯疱疹病毒1型α基因诱导所需的顺式作用位点结合。
Proc Natl Acad Sci U S A. 1987 Jan;84(1):71-5. doi: 10.1073/pnas.84.1.71.
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Comparison of upstream sequence requirements for positive and negative regulation of a herpes simplex virus immediate-early gene by three virus-encoded trans-acting factors.三种病毒编码的反式作用因子对单纯疱疹病毒立即早期基因正负调控的上游序列要求比较
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Analysis of DNA sequences which regulate the transcription of herpes simplex virus immediate early gene 3: DNA sequences required for enhancer-like activity and response to trans-activation by a virion polypeptide.单纯疱疹病毒立即早期基因3转录调控的DNA序列分析：增强子样活性及对病毒体多肽反式激活反应所需的DNA序列
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DNA sequence of the herpes simplex virus type 1 gene whose product is responsible for transcriptional activation of immediate early promoters.单纯疱疹病毒1型基因的DNA序列，其产物负责立即早期启动子的转录激活。
Nucleic Acids Res. 1985 Nov 11;13(21):7865-79. doi: 10.1093/nar/13.21.7865.
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Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.用葡萄球菌蛋白A基因融合载体固定化和纯化酶
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