Department of Chemistry and Biochemistry, Molecular Biosciences Research Group, 419 Centennial Hall, Texas State University, 601 University Drive, San Marcos, TX 78666, USA
Comp Biochem Physiol C Toxicol Pharmacol. 2012 Jan;155(1):11-7. doi: 10.1016/j.cbpc.2011.05.015. Epub 2011 Jun 1.
Aquatic hypoxia caused by organic pollution and eutrophication is a pressing worldwide water pollution problem. Better methods for monitoring oxygen levels are needed to assist efforts to maintain and protect the health of natural aquatic environments. In this project, we used a Japanese ricefish (medaka, Oryzias latipes) 8K oligonucleotide array as a platform to identify potential hypoxic biomarkers in different organs (fin, gill, liver and brain) upon exposure to hypoxia. The microarray results were validated by qRT-PCR employing a subset of candidate biomarkers. Interestingly, the largest number and most significant of hypoxia responding array features were detected in hypoxia exposed fin tissues. We identified 173 array features that exhibited a significant response (over 2 fold change in expression) upon exposure to hypoxic conditions and validated a subset of these by quantitative RT-PCR. These gene targets were subjected to annotation and gene ontology mining. Positively identifiable gene targets that may be useful for development of a rapid and accurate biomarker test using fin clips are discussed in relation to previous reports on hypoxia responsive genes.
由于有机污染和富营养化导致的水生缺氧是一个全球性的紧迫水污染问题。需要更好的方法来监测氧气水平，以协助维护和保护自然水生环境的健康。在这个项目中，我们使用了日本稻鱼（Oryzias latipes）8K 寡核苷酸阵列作为平台，以确定在缺氧暴露于不同器官（鳍、鳃、肝和脑）时的潜在缺氧生物标志物。通过 qRT-PCR 对微阵列结果进行了验证，采用了一组候选生物标志物。有趣的是，在缺氧暴露的鳍组织中检测到最多和最显著的缺氧反应阵列特征。我们鉴定了 173 个在缺氧条件下表现出显著反应（表达水平变化超过 2 倍）的阵列特征，并通过定量 RT-PCR 验证了其中的一部分。这些基因靶标进行了注释和基因本体挖掘。与先前关于缺氧反应基因的报告相关，讨论了可能有助于使用鳍夹开发快速准确的生物标志物测试的有用的可识别基因靶标。
