Ju Zhenlin, Wells Melissa C, Heater Sheila J, Walter Ronald B
Molecular Biosciences Research Group, Department of Chemistry and Biochemistry, 419 Centennial Hall, Texas State University, San Marcos, TX 78666-4616, USA.
Comp Biochem Physiol C Toxicol Pharmacol. 2007 Feb;145(1):134-44. doi: 10.1016/j.cbpc.2006.06.012. Epub 2006 Jun 30.
Due in part to human population growth watersheds and coastal estuaries have been receiving increasing run-off of nutrients and genotoxins. As a consequence, the occurrences of nutrient-driven hypoxia in coastal waters appear to be increasing. Thus, understanding the molecular genetic response to hypoxia by model aquatic organisms is of interest both from environmental and physiological viewpoints. The major objectives of this study are to determine genome-wide gene expression profiles and to better understand how hypoxia influences global gene expression in medaka (Oryzias latipes), a well utilized aquatic model species. Herein we detail our development of a microarray containing 8046 medaka unigenes and describe our experimental results for measuring gene expression changes in the brain, gill, and liver of hypoxia exposed fish. Using conservative selection criteria, we determined that 501 genes in the brain, 442 in the gill, and 715 in the liver were differentially expressed in medaka exposed to hypoxia. These differentially expressed genes fell into a number of biological gene ontology groups related to general metabolism, catabolism, RNA and protein metabolism, etc. Two biological pathways, ubiquitin-proteasome and phosphatidylinositol signaling, were significantly dysregulated in medaka upon hypoxia exposure. Comparative genomics between medaka and human identified several human orthologies associated with known diseases.
部分由于人口增长，流域和沿海河口接纳的营养物质和基因毒素径流不断增加。因此，沿海水域因营养物质导致的缺氧现象似乎在增多。所以，从环境和生理角度来看，了解模式水生生物对缺氧的分子遗传反应很有意义。本研究的主要目的是确定全基因组基因表达谱，并更好地理解缺氧如何影响青鳉（Oryzias latipes）这一广泛应用的水生模式物种的全球基因表达。在此，我们详细介绍了包含8046个青鳉单基因的微阵列的开发，并描述了测量缺氧暴露鱼的脑、鳃和肝脏中基因表达变化的实验结果。使用保守的选择标准，我们确定在暴露于缺氧的青鳉中，脑中501个基因、鳃中442个基因和肝脏中715个基因存在差异表达。这些差异表达基因分属于一些与一般代谢、分解代谢、RNA和蛋白质代谢等相关的生物学基因本体组。缺氧暴露后，青鳉体内的两条生物学途径，泛素 - 蛋白酶体途径和磷脂酰肌醇信号传导途径明显失调。青鳉和人类之间的比较基因组学确定了几个与已知疾病相关的人类直系同源基因。
