用于人类巨细胞病毒的快速抗病毒DNA-DNA杂交检测法。
Rapid antiviral DNA-DNA hybridization assay for human cytomegalovirus.
作者信息
Dankner W M, Scholl D, Stanat S C, Martin M, Sonke R L, Spector S A
机构信息
Department of Pediatrics, University of California, San Diego.
出版信息
J Virol Methods. 1990 Jun;28(3):293-8. doi: 10.1016/0166-0934(90)90122-v.
A rapid DNA-DNA hybridization technique that can be accomplished in 4 to 5 days was compared with plaque reduction assay to determine its reliability in performing antiviral assays for human cytomegalovirus (HCMV). The assay involves lysing infected cells, direct wicking of denatured DNA onto membranes and hybridization using a 125I-labeled HCMV DNA probe. Using ten ganciclovir sensitive clinical HCMV strains for comparison, the DNA hybridization technique correlated well with the plaque assay. Clinical HCMV strains previously identified as resistant to ganciclovir were also readily identified. The DNA-DNA hybridization assay is less tedious and more rapid than plaque reduction assays, and thus, provides an excellent alternative for evaluation of the antiviral activity of drugs against HCMV.
一种可在4至5天内完成的快速DNA-DNA杂交技术与蚀斑减少试验进行了比较，以确定其在进行人巨细胞病毒（HCMV）抗病毒试验中的可靠性。该试验包括裂解感染细胞、将变性DNA直接芯吸到膜上以及使用125I标记的HCMV DNA探针进行杂交。使用十种对更昔洛韦敏感的临床HCMV毒株进行比较，DNA杂交技术与蚀斑试验相关性良好。先前鉴定为对更昔洛韦耐药的临床HCMV毒株也很容易被鉴定出来。DNA-DNA杂交试验比蚀斑减少试验更不繁琐且更快速，因此，为评估药物对HCMV的抗病毒活性提供了一种极佳的替代方法。
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